Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme without pro-domain and of the isolated MMP-2 CBD domain in Escherichia coli in inclusion bodies | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | inhibition of MMP-2 gelatinolysis by targeting exodomain-substrate interactions | Homo sapiens | |
peptide P713 | inhibits the binding of the CBD as well as MMP-2E404A to gelatin, no inhibition of MMP-2 lacking thr CBD domain, competitively, P713 inhibits MMP-2 activities by blocking substrate access to the CBD exodomain, mechanism, overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
collagene type IV + H2O | Homo sapiens | - |
? | - |
? | |
Gelatin + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | artificial biotinylation of the purified recombinant CBD domain for library screening, overview | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme without pro-domain and isolated MMP-2 CBD domain from Escherichia coli inclusion bodies, after solubilization and refolding, by nickel affinity chromatography | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
solubilization of recombinant His-tagged enzyme without pro-domain and isolated MMP-2 CBD domain from Escherichia coli inclusion bodies with 8 M urea, 0.1 M NaH2PO4 and 0.01 M Tris-HCl, pH 8.0, followed by refolding | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl)acetyl-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Lys(2,4-dinitrophenyl)-Gly + H2O | a short 11-amino-acid collagen-like peptide substrate, NFF-1, cleavage of NFF-1 by MMP-2 does not require CBD-mediated substrate binding for degradation to occur | Homo sapiens | ? | - |
? | |
collagene type IV + H2O | - |
Homo sapiens | ? | - |
? | |
collagene type IV + H2O | DQ-collagen type IV zymography | Homo sapiens | ? | - |
? | |
Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
Gelatin + H2O | gelatin zymography | Homo sapiens | ? | - |
? | |
additional information | MMP-2 CBD-binding peptides from a random peptide library screening using biotinylated recombinant CBD domain, and interactions of immobilized synthetic peptides with the recombinant CBD, MMP-2E404A, MMP-2DELTACBD and AlkCBD, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Matrix metalloproteinase 2 | - |
Homo sapiens |
MMP-2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at, fluorogenic peptide substrate | Homo sapiens |
7.6 | - |
assay at, gelatin or collagen IV zymography | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
pH 7.0, 22°C | Homo sapiens | peptide P713 |