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Literature summary for 3.4.24.24 extracted from
- Identification, purification and characterization of matrix metalloproteinase-2 in bovine pulmonary artery smooth muscle plasma membrane (2004), Mol. Cell. Biochem., 258, 73-89.
General Stability
| General Stability | Organism |
|---|---|
| stable in aqueous solution | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Bos taurus |  |
| alpha2 macroglobulin | - |
Bos taurus | |
| EDTA | - |
Bos taurus | |
| EGTA | - |
Bos taurus | |
| Tissue inhibitor of metalloproteinase-2 | - |
Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Bos taurus | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required, optimal concentration 10 mM | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 72000, proenzyme, x * 62000 and x * 45000, active species, SDS-PAGE | Bos taurus |
| 62000 | - |
x * 72000, proenzyme, x * 62000 and x * 45000, active species, SDS-PAGE | Bos taurus |
| 72000 | - |
x * 72000, proenzyme, x * 62000 and x * 45000, active species, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | activation of 72 kDa proenzyme by aminophenyl-mercuric acetate to 62 and 45 kDa species, no spontaneous autoactivation | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from pulmonary artery smooth muscle plasma membrane | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| smooth muscle | pulmonary artery smooth muscle | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1045 | - |
37°C | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4-dinitrophenyl-L-Pro-L-Gln-Gly-L-Ile-L-Ala-Gly-L-Gln-D-Arg + H2O | - |
Bos taurus | ? | - |
? | |
| collagen type IV + H2O | - |
Bos taurus | ? | - |
? | |
| collagen type V + H2O | - |
Bos taurus | ? | - |
? | |
| gelatin type I + H2O | - |
Bos taurus | ? | - |
? | |
| gelatin type IV + H2O | - |
Bos taurus | ? | - |
? | |
| gelatin type V + H2O | - |
Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 72000, proenzyme, x * 62000 and x * 45000, active species, SDS-PAGE | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Bos taurus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bos taurus | isoelectric focusing | 6.4 | 6.2 |
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Release 2023.1 (January 2023)
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