Literature summary for 3.4.24.24 extracted from

Olson, M.W.; Gervasi, D.C.; Mobashery, S.; Fridman, R.
Kinetic analysis of the binding of human matrix metalloproteinase-2 and -9 to tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 (1997), J. Biol. Chem., 272, 29975-29983.

Search for more literature for 3.4.24.24

Inhibitors

Inhibitors	Comment	Organism	Structure
TIMP-1	-	Homo sapiens
TIMP-2	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00152	-	MOAcPLGLA2pr(Dnp)-AR-NH2	pH 8.0, 25°C, 45 kDa form	Homo sapiens
0.00306	-	MOAcPLGLA2pr(Dnp)-AR-NH2	pH 8.0, 25°C, 62 kDa form	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	active form lacking the C-terminal domain, SDS-PAGE	Homo sapiens
62000	-	SDS-PAGE	Homo sapiens
72000	-	SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	human	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
MOAcPLGLA2pr(Dnp)-AR-NH2 + H2O	fluorogenic quenching substrate	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
gelatinase A	-	Homo sapiens
MMP-2	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.3	-	MOAcPLGLA2pr(Dnp)-AR-NH2	pH 8.0, 25°C, 45 kDa form	Homo sapiens
3	6	MOAcPLGLA2pr(Dnp)-AR-NH2	pH 8.0, 25°C, 62 kDa form	Homo sapiens
3.18	-	MOAcPLGLA2pr(Dnp)-AR-NH2	pH 8.0, 25°C, 62 kDa form	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000072	-	TIMP-2	pH 8.0, 25°C, 62 kDa form	Homo sapiens
0.0000097	-	TIMP-1	pH 8.0, 25°C, 62 kDa form	Homo sapiens
0.000275	-	TIMP-2	pH 8.0, 25°C, 45 kDa form	Homo sapiens

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Release 2023.1 (January 2023)