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Literature summary for 3.4.24.24 extracted from

  • Okada, Y.; Morodomi, T.; Enghild, J.J.; Suzuki, K.; Yasui, A.; Nakanishi, I.; Salvesen, G.; Nagase, H.
    Matrix metalloproteinase 2 from human rheumatoid synovial fibroblasts. Purification and activation of the precursor and enzymic properties (1990), Eur. J. Biochem., 194, 721-730.
    View publication on PubMed
Search for more literature for 3.4.24.24

Activating Compound

Activating Compound Comment Organism Structure
additional information rapid activation of the zymogen by 4-aminophenylmercuric acetate Homo sapiens
additional information no activation by endopeptidases (trypsin, chymotrypsin, plasmin, plasma kallikrein, thrombin, neutrophil elastase, cathepsin G, matrix metalloproteinase 3, thermolysin) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Homo sapiens
1,4-dithiothreitol
-
 Homo sapiens
EDTA
-
 Homo sapiens
glycine
-
 Homo sapiens
additional information not: inhibitors of serine, cysteine or aspartic proteinases Homo sapiens
SDS
-
 Homo sapiens
Tissue inhibitor of metalloproteinases
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 x * 67000, human, active form, SDS-PAGE under reducing condition Homo sapiens
72000
-
 x * 72000, human, zymogen, SDS-PAGE under reducing conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens constitutively expressed in human rheumatoid synovial cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
as an inactive zymogen, rapid activation by 4-aminophenylmercuric acetate Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
culture medium from human rheumatoid synovial cells Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Azocoll + H2O
-
 Homo sapiens ?
-
 ?
Cartilage proteoglycan + H2O
-
 Homo sapiens ?
-
 ?
Collagen + H2O type V Homo sapiens ?
-
 ?
Collagen + H2O type IV (to a lesser extent) Homo sapiens ?
-
 ?
Elastin + H2O
-
 Homo sapiens ?
-
 ?
Fibronectin + H2O
-
 Homo sapiens ?
-
 ?
Gelatin + H2O
-
 Homo sapiens ?
-
 ?
Laminin + H2O
-
 Homo sapiens ?
-
 ?
additional information constitutively expressed in human rheumatoid synovial cells Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 72000, human, zymogen, SDS-PAGE under reducing conditions Homo sapiens
? x * 67000, human, active form, SDS-PAGE under reducing condition Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 azocoll Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 40% of activity maximum, pH 9.0: about 85% of activity maximum, azocoll Homo sapiens