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Literature summary for 3.4.24.22 extracted from
- Human stromelysins 1 and 2 (1995), Methods Enzymol., 248, 449-470.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Homo sapiens |  |
| DTT | - |
Homo sapiens | |
| EDTA | - |
Homo sapiens | |
| EGTA | - |
Homo sapiens | |
| Specific tissue inhibitor of metalloproteinases | TIMP-1 | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
(7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | zinc metalloenzyme | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
synthesized as preproenzyme of 476 amino acids and secreted from cells as the proenzyme | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | participates in activation of procollagenase proMMP-1 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | potential glycosylation site at Asn100, but is unlikely to be a glycoprotein as it does not bind concanavalin A | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, prostromelysin 2, 50 mM Tris-HCl, pH 7.5, 0.15 M NaCl, 10 mM CaCl2, 0.02% w/v NaN3, 0.05% Brij 35, stable for 4 weeks | Homo sapiens |
| 4°C, prostromelysin 2, 50 mM Tris-HCl, pH 7.5, 0.15 M NaCl, 10 mM CaCl2, 0.02% w/v NaN3, 0.05% Brij 35, stable for 2 weeks | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 + H2O | - |
Homo sapiens | (7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu + norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 | - |
? | |
| Aggrecan core protein + H2O | - |
Homo sapiens | ? | - |
? | |
| Azocoll + H2O | - |
Homo sapiens | ? | - |
? | |
| Cartilage link protein + H2O | cleaved at His16-Ile17 bond | Homo sapiens | ? | - |
? | |
| Collagen + H2O | type IV | Homo sapiens | ? | - |
? | |
| Elastin + H2O | - |
Homo sapiens | ? | - |
? | |
| Fibronectin + H2O | negligible | Homo sapiens | ? | - |
? | |
| Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
| additional information | participates in activation of procollagenase proMMP-1 | Homo sapiens | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.8 | - |
(7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 | - |
Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
activity against aggrecan at pH 5.5 is double that at pH 6.0 | Homo sapiens |
| 7.5 | 8 | azocoll | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | 6.0: 25% of activity maximum, 7.5-8.0: activity maximum, azocoll | Homo sapiens |
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