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Literature summary for 3.4.24.21 extracted from

  • Guevara, T.; Yiallouros, I.; Kappelhoff, R.; Bissdorf, S.; Stoecker, W.; Gomis-Rueth, F.X.
    Proenzyme structure and activation of astacin metallopeptidase (2010), J. Biol. Chem., 285, 13958-13965.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Astacus astacus

Crystallization (Commentary)

Crystallization (Comment) Organism
proastacin is crystallized by the sitting drop vapor diffusion method, using 20% (w/v) polyethylene glycol 8000, 0.1 M (NH4)2SO4, 0.01 M MgCl2, 0.05 M MES, pH 5.6 Astacus astacus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required for activity Astacus astacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen + H2O Astacus astacus
-
 ?
-
 ?
Gelatin + H2O Astacus astacus
-
 ?
-
 ?
additional information Astacus astacus optimal astacin substrates comprise at least five amino acids with small aliphatic residues in P'1, proline in P'2, bulky hydrophobic residues in P'3, and basic residues in P1 and P2 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Astacus astacus Q9U918 European crayfish
-

Purification (Commentary)

Purification (Comment) Organism
proastacin is purified by Ni-NTA column chromatography and HEMA-Bio gel filtration Astacus astacus

Source Tissue

Source Tissue Comment Organism Textmining
midgut gland astacin is synthesized as a preproenzyme in the midgut gland Astacus astacus
-
pancreatic duct the proenzyme is only transiently found within the ducts between the hepatopancreas and the stomach Astacus astacus
-
stomach mature enzyme Astacus astacus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Collagen + H2O
-
 Astacus astacus ?
-
 ?
Gelatin + H2O
-
 Astacus astacus ?
-
 ?
additional information optimal astacin substrates comprise at least five amino acids with small aliphatic residues in P'1, proline in P'2, bulky hydrophobic residues in P'3, and basic residues in P1 and P2 Astacus astacus ?
-
 ?