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Literature summary for 3.4.24.21 extracted from

  • Wolz, R.L.
    A kinetic comparison of the homologous proteases astacin and meprin A (1994), Arch. Biochem. Biophys., 310, 144-151.
    View publication on PubMed
Search for more literature for 3.4.24.21

Inhibitors

Inhibitors Comment Organism Structure
Acetyl-Arg-Pro-Gly-Tyr-hydroxamate
-
 Astacus astacus
actinonin i.e. 3-[[1-[[2-(hydroxymethyl)-1-pyrolidinyl]carbonyl]-2-methylpropyl]carbamoyl]octano hydroxamic acid, weak, kinetics Astacus astacus
Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg nitrobradykinin as substrate Astacus astacus
Arg-Pro-Pro-Gly-(4-nitro)Phe-Glu-Pro-Phe-Arg nitrobradykinin as substrate Astacus astacus
Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg nitrobradykinin as substrate Astacus astacus
Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg nitrobradykinin as substrate Astacus astacus
Hydroxamylsuccinyl-Pro-Phe-Arg
-
 Astacus astacus
additional information most effective inhibitors are hydroxamates with aromatic side chains followed in order of decreasing affinity by those with side chains containing sulfur, acidic or basic groups Astacus astacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 2-Aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
0.044
-
 2-Aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
0.085
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg
-
 Astacus astacus
0.116
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg
-
 Astacus astacus
0.154
-
 2-Aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
0.194
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg
-
 Astacus astacus
0.209
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg
-
 Astacus astacus
0.281
-
 2-Aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
0.306
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg
-
 Astacus astacus
0.327
-
 2-Aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
0.867 0.87 2-Aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
0.954
-
 2-Aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ requirement Astacus astacus
Zn2+ zinc metalloenzyme Astacus astacus

Organism

Organism UniProt Comment Textmining
Astacus astacus
-
 i.e. Astacus fluviatilis
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Phe-Ser Astacus astacus 2-aminobenzoyl-Arg-Gly-Pro-Phe + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
2-aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Phe-Ser Astacus astacus 2-aminobenzoyl-Arg-Hyp-Gly-Phe + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
2-aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage sites: Gly-Ala and Ala-Ser Astacus astacus 2-aminobenzoyl-Arg-Pro-Gly-Ala + Ser-Pro-(4-nitro)Phe-Arg major products ?
2-aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Glu-Ser Astacus astacus 2-aminobenzoyl-Arg-Pro-Gly-Glu + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
2-aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Leu-Ser Astacus astacus 2-aminobenzoyl-Arg-Pro-Gly-Leu + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
2-aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg + H2O fluorogenic bradykinin analog substrate, cleavage site: Lys-Ser Astacus astacus 2-aminobenzoyl-Arg-Pro-Gly-Lys + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
2-aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg + H2O best of fluorogenic bradykinin analog substrates, cleavage site: Phe-Ser Astacus astacus 2-aminobenzoyl-Arg-Pro-Ile-Phe + Ser-Pro-(4-nitro)Phe-Arg
-
 ?
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg + H2O chromogenic bradykinin analog Astacus astacus Arg-Pro-Pro-Gly-(4-nitro)Phe + Ala-Pro-Phe-Arg
-
 ?
Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg + H2O chromogenic bradykinin analog Astacus astacus Arg-Pro-Pro-Gly-(4-nitro)Phe + Arg-Pro-Phe-Arg
-
 ?
Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg + H2O chromogenic bradykinin analog Astacus astacus Arg-Pro-Pro-Gly-(4-nitro)Phe + Lys-Pro-Phe-Arg
-
 ?
Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg + H2O chromogenic bradykinin analog Astacus astacus Arg-Pro-Pro-Gly-(4-nitro)Phe + Phe-Pro-Phe-Arg
-
 ?
Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg + H2O i.e. nitrobradykinin, chromogenic bradykinin analog Astacus astacus Arg-Pro-Pro-Gly-(4-nitro)Phe + Ser-Pro-Phe-Arg
-
 ?
azocasein + H2O
-
 Astacus astacus ?
-
 ?
bradykinin + H2O i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg Astacus astacus Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg
-
 ?
additional information No hydrolysis of Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg, Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg, Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg, Arg-Pro-Pro-Gly-(4-nitro)Phe-Glu-Pro-Phe-Arg Astacus astacus ?
-
 ?
Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O
-
 Astacus astacus Succinyl-Ala-Ala-Ala + p-nitroaniline
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Astacus astacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Lys-Pro-Phe-Arg
-
 Astacus astacus
0.002
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Arg-Pro-Phe-Arg
-
 Astacus astacus
0.004
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Phe-Pro-Phe-Arg
-
 Astacus astacus
2.8
-
 2-aminobenzoyl-Arg-Pro-Gly-Glu-Ser-Pro-4-nitrophenylalanine-Arg
-
 Astacus astacus
3.6
-
 2-Aminobenzoyl-Arg-Pro-Gly-Lys-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
7.1
-
 2-Aminobenzoyl-Arg-Gly-Pro-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
14.3
-
 2-Aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
15.1
-
 2-Aminobenzoyl-Arg-Hyp-Gly-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
44.4
-
 2-Aminobenzoyl-Arg-Pro-Gly-Ala-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
78.4
-
 2-Aminobenzoyl-Arg-Pro-Ile-Phe-Ser-Pro-(4-nitro)Phe-Arg
-
 Astacus astacus
78.7
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ser-Pro-Phe-Arg
-
 Astacus astacus
834
-
 Arg-Pro-Pro-Gly-(4-nitro)Phe-Ala-Pro-Phe-Arg
-
 Astacus astacus