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Literature summary for 3.4.24.20 extracted from

  • Nonaka, T.; Hashimoto, Y.; Takio, K.
    Kinetic characterization of lysine-specific metalloendopeptidases from Grifola frondosa and Pleurotus ostreatus fruiting bodies (1998), J. Biochem., 124, 157-162.
    View publication on PubMed
Search for more literature for 3.4.24.20

Inhibitors

Inhibitors Comment Organism Structure
2,4-dinitrophenyl-Ala-Thr-D-lysine-Leu-Ser-Try
-
 Grifola frondosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0088
-
 2,4-dinitrophenyl-DATKLSW pH 9.5, 37°C Grifola frondosa
0.0111
-
 2,4-dinitrophenyl-APKLSW pH 9.5, 37°C Grifola frondosa
0.0114
-
 2,4-dinitrophenyl-STATKLSW pH 9.5, 37°C Grifola frondosa
0.0116
-
 2,4-dinitrophenyl-ADKLSW pH 9.5, 37°C Grifola frondosa
0.0121
-
 2,4-dinitrophenyl-ATKLSW pH 9.5, 37°C Grifola frondosa
0.0133
-
 2,4-dinitrophenyl-ATKLSTSW pH 9.5, 37°C Grifola frondosa
0.0152
-
 2,4-dinitrophenyl-ARKLSW pH 9.5, 37°C Grifola frondosa
0.0155
-
 2,4-dinitrophenyl-DADKLSW pH 9.5, 37°C Grifola frondosa
0.0177
-
 2,4-dinitrophenyl-ATKRSW pH 9.5, 37°C Grifola frondosa
0.0249
-
 2,4-dinitrophenyl-ATKDSW pH 9.5, 37°C Grifola frondosa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can substitute for Zn2+, enhances activity Pleurotus ostreatus
Zn2+ zinc-metalloendopetidase Grifola frondosa
Zn2+ zinc-metalloendopetidase Pleurotus ostreatus

Organism

Organism UniProt Comment Textmining
Grifola frondosa P81054 edible mushroom
-
Pleurotus ostreatus P81055 edible mushroom
-

Purification (Commentary)

Purification (Comment) Organism
-
 Grifola frondosa
-
 Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenyl-ADKLSW + H2O
-
 Grifola frondosa ?
-
 ?
2,4-dinitrophenyl-APKLSW + H2O
-
 Grifola frondosa ?
-
 ?
2,4-dinitrophenyl-APKLSW + H2O
-
 Grifola frondosa 2,4-dinitrophenyl-Ala-Pro + KLSW
-
 ?
2,4-dinitrophenyl-ARKLSW + H2O
-
 Grifola frondosa ?
-
 ?
2,4-dinitrophenyl-ARKLSW + H2O
-
 Grifola frondosa 2,4-dinitrophenyl-Ala-Arg + KLSW
-
 ?
2,4-dinitrophenyl-ATKDSW + H2O
-
 Grifola frondosa ?
-
 ?
2,4-dinitrophenyl-ATKDSW + H2O
-
 Grifola frondosa 2,4-dinitrophenyl-Ala-Thr + KDSW
-
 ?
2,4-dinitrophenyl-ATKLSTSW + H2O
-
 Grifola frondosa ?
-
 ?
2,4-dinitrophenyl-ATKLSTSW + H2O
-
 Grifola frondosa 2,4-dinitrophenyl-Ala-Thr + KLSTSW
-
 ?
2,4-dinitrophenyl-ATKLSW + H2O
-
 Grifola frondosa ?
-
 ?
2,4-dinitrophenyl-ATKLSW + H2O
-
 Grifola frondosa 2,4-dinitrophenyl-Ala-Thr + KLSW
-
 ?
2,4-dinitrophenyl-ATKRSW + H2O
-
 Grifola frondosa ?
-
 ?
2,4-dinitrophenyl-ATKRSW + H2O
-
 Grifola frondosa 2,4-dinitrophenyl-Ala-Thr + KRSW
-
 ?
2,4-dinitrophenyl-DADKLSW + H2O
-
 Grifola frondosa ?
-
 ?
2,4-dinitrophenyl-DADKLSW + H2O
-
 Grifola frondosa 2,4-dinitrophenyl-DAD + KLSW
-
 ?
2,4-dinitrophenyl-DATKLSW + H2O
-
 Grifola frondosa ?
-
 ?
2,4-dinitrophenyl-DATKLSW + H2O
-
 Grifola frondosa 2,4-dinitrophenyl-DAT + KLSW
-
 ?
2,4-dinitrophenyl-Ser-Thr-Ala-Thr-Lys-Leu-Ser-Trp + H2O fluorescent peptide substrate Grifola frondosa ?
-
 ?
2,4-dinitrophenyl-Ser-Thr-Ala-Thr-Lys-Leu-Ser-Trp + H2O fluorescent peptide substrate Pleurotus ostreatus ?
-
 ?
2,4-dinitrophenyl-STATKLSW + H2O
-
 Grifola frondosa ?
-
 ?
2,4-dinitrophenyl-STATKLSW + H2O
-
 Grifola frondosa 2,4-dinitrophenyl-STAT + KLSW
-
 ?
azocasein + H2O
-
 Grifola frondosa ?
-
 ?
azocasein + H2O
-
 Pleurotus ostreatus ?
-
 ?
additional information peptides with aspartic acid adjacent to the Lys residue are poor substrates, 2,4-dinitrophenyl-Thr-Lys-Trp and peptides with substitution of L-Arg, L-ornithine, or D-Lys are no substrates Grifola frondosa ?
-
 ?
additional information peptides with aspartic acid adjacent to the Lys residue are poor substrates, 2,4-dinitrophenyl-Thr-Lys-Trp and peptides with substitution of L-Arg, L-ornithine, or D-Lys are no substrates Pleurotus ostreatus ?
-
 ?

Synonyms

Synonyms Comment Organism
GFMEP
-
 Grifola frondosa
POMEP
-
 Pleurotus ostreatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 2,4-dinitrophenyl-DADKLSW pH 9.5, 37°C Grifola frondosa
0.25
-
 2,4-dinitrophenyl-ATKDSW pH 9.5, 37°C Grifola frondosa
0.99
-
 2,4-dinitrophenyl-ATKLSTSW pH 9.5, 37°C Grifola frondosa
1.3
-
 2,4-dinitrophenyl-ADKLSW pH 9.5, 37°C Grifola frondosa
3
-
 2,4-dinitrophenyl-ATKLSW pH 9.5, 37°C Grifola frondosa
3.7
-
 2,4-dinitrophenyl-ATKRSW pH 9.5, 37°C Grifola frondosa
5.1
-
 2,4-dinitrophenyl-DATKLSW pH 9.5, 37°C Grifola frondosa
5.5
-
 2,4-dinitrophenyl-APKLSW pH 9.5, 37°C Grifola frondosa
17.6
-
 2,4-dinitrophenyl-ATKLSTSW pH 9.5, 37°C Grifola frondosa
21
-
 2,4-dinitrophenyl-STATKLSW pH 9.5, 37°C Grifola frondosa
54.7
-
 2,4-dinitrophenyl-ARKLSW pH 9.5, 37°C Grifola frondosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
 azocasein hydrolysis Pleurotus ostreatus
8.5
-
 rather active with fluorogenic peptide substrates than at pH 5.0 Pleurotus ostreatus
9.5
-
-
 Grifola frondosa

pH Range

pH Minimum pH Maximum Comment Organism
6 10.5 exhibits more than 50% of the maximal activity over this range Grifola frondosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0399
-
 2,4-dinitrophenyl-Ala-Thr-D-lysine-Leu-Ser-Try pH 9.5, 37°C Grifola frondosa