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Literature summary for 3.4.24.20 extracted from

  • Lewis, W.G.; Basford, J.M.; Wlatoon, P.L.
    Specificity and inhibition studies of Armillaria mellea protease (1978), Biochim. Biophys. Acta, 522, 551-560.
    View publication on PubMed
Search for more literature for 3.4.24.20

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Armillaria mellea
2,2'-bipyridine
-
 Armillaria mellea
ethylamine
-
 Armillaria mellea
imidazole
-
 Armillaria mellea
L-arginine weak Armillaria mellea
L-lysine
-
 Armillaria mellea
L-ornithine weak Armillaria mellea
methylamine
-
 Armillaria mellea
n-Propylamine
-
 Armillaria mellea
S-2-aminoethylcysteine
-
 Armillaria mellea

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc requirement, 1 atom per molecule of native enzyme Armillaria mellea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Armillaria mellea
13500
-
 1 * 13500, Armillaria mellea, SDS-PAGE Armillaria mellea
14000
-
 Armillaria mellea, gel filtration Armillaria mellea
16650
-
 Armillaria mellea, most probable MW deduced from amino acid composition Armillaria mellea

Organism

Organism UniProt Comment Textmining
Armillaria mellea
-
 basidomycete, honey fungus
-

Purification (Commentary)

Purification (Comment) Organism
-
 Armillaria mellea

Source Tissue

Source Tissue Comment Organism Textmining
pileus
-
 Armillaria mellea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Armillaria mellea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Azocoll + H2O general protease substrate Armillaria mellea ?
-
 ?
Glucagon + H2O cleavage site: Ser11-Lys12, 2 products Armillaria mellea ?
-
 ?
additional information major peptide bond specificity of Armillaria mellea enzyme towards bonds where Lys residue contributes an alpha-amino group Armillaria mellea ?
-
 ?
Oxidized ribonuclease A + H2O cleavage sites: -Xaa-Lys-, multiple products Armillaria mellea ?
-
 ?
S-Carboxymethyl lysozyme + H2O cleavage sites: -Xaa-Lys-, multiple products Armillaria mellea ?
-
 ?
S-Carboxymethyl pepsinogen + H2O cleavage sites: -Xaa-Lys-, 9 peptide products Armillaria mellea ?
-
 ?
Vasopressin + H2O lysine-vasopressin Armillaria mellea ?
-
 ?
Vasopressin + H2O native and oxidized form, cleavage site: Pro7-Lys8, 2 product peptides Armillaria mellea ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 13500, Armillaria mellea, SDS-PAGE Armillaria mellea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Armillaria mellea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 broad, azocoll as substrate Armillaria mellea

pH Range

pH Minimum pH Maximum Comment Organism
4.8 9.3 about half-maximal activity at pH 4.8 and 9.3, azocoll as substrate Armillaria mellea