General Stability | Organism |
---|---|
Solubilized PPH is rather labile, immobilization by protein A-Sepharose stabilizes | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Homo sapiens | |
2-(N-Hydroxycarboxamido)-4-methylpentanoyl-L-alanyl-glycylamide | i.e. Zinkov inhibitor | Homo sapiens | |
Ca2+ | above 1 mM, metalloproteinase, does not require additional Ca2+ | Homo sapiens | |
captopril | - |
Homo sapiens | |
DTT | - |
Homo sapiens | |
EDTA | restored by divalent metal ions, Zn2+ being more effective than Ca2+ or Mg2+ | Homo sapiens | |
additional information | amastatin, bestatin, puromycin, N-tosyl-L-Phe chloromethyl ketone, monoclonal antibody HBB 3/716/36; iodoacetate; leupeptin; no inhibition by phosphoramidon; PMSF, pepstatin | Homo sapiens | |
Zn2+ | above 1 mM, metalloproteinase, does not require additional Zn2+ | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | apical membrane of intestinal epithelial cells | Homo sapiens | 16020 | - |
microvillus | - |
Homo sapiens | 5902 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Calcium | metalloendopeptidase | Homo sapiens | |
Calcium | Ca2+ inhibits above 1 mM | Homo sapiens | |
Zinc | Zn2+ inhibits above 1 mM | Homo sapiens | |
Zinc | metalloendopeptidase | Homo sapiens | |
Zinc | zinc binding motif | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
2 * 100000, human, SDS-PAGE in the presence of DTT | Homo sapiens |
200000 | - |
human, SDS-PAGE, non-reducing conditions | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
PPH | - |
Homo sapiens PPH | - |
PPH | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | highly glycosylated | Homo sapiens |
Purification (Comment) | Organism |
---|---|
from partially purified microvillar membranes | Homo sapiens |
immunoaffinity chromatography | Homo sapiens |
to near homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | higher specific activity in distal ileum than in proximal duodenum | Homo sapiens | - |
Storage Stability | Organism |
---|---|
4°C, immobilized enzyme, 3 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin I + H2O | 2 cleavage sites: Tyr-Ile and Phe-His | Homo sapiens | Asp-Arg-Val-Tyr + Ile-His-Pro-Phe + Ile-His-Pro-Phe-His-Leu | - |
? | |
angiotensin I + H2O | i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, cleavage site: Tyr-Ile | Homo sapiens | Asp-Arg-Val-Tyr + Ile-His-Pro-Phe + Ile-His-Pro-Phe-His-Leu | - |
? | |
angiotensin II + H2O | cleavage site: Tyr-Ile | Homo sapiens | Asp-Arg-Val-Tyr + Ile-His-Pro-Phe | - |
? | |
angiotensin II + H2O | i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe | Homo sapiens | Asp-Arg-Val-Tyr + Ile-His-Pro-Phe | - |
? | |
azocasein + H2O | - |
Homo sapiens | fragments of azocasein | - |
? | |
azocasein + H2O | - |
Homo sapiens PPH | fragments of azocasein | - |
? | |
benzoyl-Gly-His-Leu + H2O | very poor substrate, t1/2 of more than 16 h | Homo sapiens | ? | - |
? | |
benzoyl-Gly-His-Leu + H2O | very poor substrate, t1/2 of more than 16 h | Homo sapiens PPH | ? | - |
? | |
bradykinin + H2O | i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, bradykinin(1-7) and bradykinin(1-8) best substrates next to substance P | Homo sapiens | Arg-Pro-Pro-Gly + Phe-Ser-Pro-Phe-Arg | - |
? | |
bradykinin + H2O | one cleavage site: Phe-Ser | Homo sapiens | Arg-Pro-Pro-Gly + Phe-Ser-Pro-Phe-Arg | - |
? | |
additional information | hydrolysis occurs on carboxy side of aromatic residues | Homo sapiens | ? | - |
? | |
additional information | dansyl-(D)-Ala-Gly-4-phenylalanylglycine, [Leu]-enkephalin, [Met]-enkephalin | Homo sapiens | ? | - |
? | |
additional information | hydrolysis occurs on carboxy side of aromatic residues | Homo sapiens PPH | ? | - |
? | |
additional information | dansyl-(D)-Ala-Gly-4-phenylalanylglycine, [Leu]-enkephalin, [Met]-enkephalin | Homo sapiens PPH | ? | - |
? | |
N-Benzoyl-L-tyrosyl-4-aminobenzoate + H2O | very poor substrate, t1/2 of more than 16 h | Homo sapiens | N-Benzoyl-L-tyrosine + 4-aminobenzoate | - |
? | |
N-Benzoyl-L-tyrosyl-4-aminobenzoate + H2O | very poor substrate, t1/2 of more than 16 h | Homo sapiens PPH | N-Benzoyl-L-tyrosine + 4-aminobenzoate | - |
? | |
Oxytocin + H2O | poor substrate | Homo sapiens | Hydrolyzed oxytocin | - |
? | |
Substance P + H2O | best substrate | Homo sapiens | Hydrolyzed substance P | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 100000, human, SDS-PAGE in the presence of DTT | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
marked microheterogeneity due to glycosylation with pIs in the range of 6-6.85 | Homo sapiens |
7.5 | 8 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | plateau with maximal activity at pH 7.5-8 | Homo sapiens |