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Literature summary for 3.4.24.18 extracted from
- Purification and characterization of a metallo-endoproteinase from mouse kidney (1981), Biochem. J., 199, 591-598.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Mus musculus |  |
| 2-mercaptoethanol | - |
Mus musculus | |
| chymostatin | - |
Mus musculus | |
| cysteine | not cystine | Mus musculus | |
| dithioerythritol | - |
Mus musculus | |
| EDTA | - |
Mus musculus | |
| EGTA | - |
Mus musculus | |
| GSH | not oxidized form | Mus musculus | |
| additional information | diisopropyl phosphofluoridate, p-hydroxymercuribenzoate, phenylmercurisulfonate, antipain, cystine, oxidized glutathione; iodoacetate; leupeptin; no inhibition by phosphoramidon; PMSF, pepstatin | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Mus musculus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | considerable stimulation of azocasein hydrolysis at ionic strength values up to 0.15-0.2 | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81000 | - |
x * 81000, mouse, SDS-PAGE in the presence of 2-mercaptoethanol | Mus musculus |
| 270000 | 320000 | mouse, gel filtration | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Mus musculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| solubilized from 100000 g sediment with toluene and trypsin | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Aldolase + H2O | - |
Mus musculus | ? | - |
? | |
| azocasein + H2O | - |
Mus musculus | fragments of azocasein | - |
? | |
| Azocoll + H2O | poor substrate | Mus musculus | ? | - |
? | |
| Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O | - |
Mus musculus | ? | - |
? | |
| casein + H2O | - |
Mus musculus | ? | - |
? | |
| Hemoglobin + H2O | poor substrate | Mus musculus | ? | - |
? | |
| L-Leu 2-naphthylamide + H2O | - |
Mus musculus | ? | - |
? | |
| additional information | little or no activity towards benzoylarginine 2-naphthylamide, benzoylglycylarginine, benzyloxycarbonyl-Glu-Tyr, acetyl-Phe 2-naphthylester | Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 81000, mouse, SDS-PAGE in the presence of 2-mercaptoethanol | Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 4.3 | Mus musculus |
| 9.5 | - |
azocasein as substrate | Mus musculus |
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