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Literature summary for 3.4.24.17 extracted from
- A metalloproteinase from human rheumatoid synovial fibroblasts that digests connective tissue matrix components. Purification and characterization (1986), J. Biol. Chem., 261, 14245-14255.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Homo sapiens |  |
| EDTA | - |
Homo sapiens | |
| additional information | not: inhibitors for serine, cysteine or aspartic proteinases | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | removal of Ca2+ from the enzyme solution results in a complete loss of activity that can be fully restored by addition of 1 mM Ca2+ | Homo sapiens | |
| Co2+ | addition of 0.5 mM Co2+ in the presence of Ca2+ restores activity of the apoenzyme, not by Co2+ alone | Homo sapiens | |
| Mn2+ | addition of 5 mM Mn2+ in the presence of Ca2+ restores activity of the apoenzyme, not by Mn2+ alone | Homo sapiens | |
| Zinc | Zn2+: addition of 0.5 mM Zn2+ in the presence of Ca2+ restores activity of the apoenzyme, not by Zn2+ alone | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
human, latent precursor, MW 51000, gel filtration | Homo sapiens |
| 28000 | - |
x * 28000, human, form LMW, SDS-PAGE | Homo sapiens |
| 45000 | - |
x * 45000, human, form HMW, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | may play a role in the normal turnover of the connective tissue matrix as well as in the joint destruction of chronic synovitis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 active forms: HMW, LMW | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | of rheumatoid synovial cells | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0°C or 4°C, 50 mM Tris-HCl, pH 7.5, 0.4 M NaCl, 10 mM Ca2+, 0.05% Brij 35, 0.02% NaN3, less than 19% loss of activity after 10 weeks | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Collagen type IV + H2O | - |
Homo sapiens | More | 4 major fragments of MW 165000, 145000, 125000 and 110000 | ? | |
| Fibronectin + H2O | - |
Homo sapiens | Intact fibronectin subunit MW 22000 + a disulfide-bonded COOH-terminal MW 20000 polypeptide | - |
? | |
| Gelatin + H2O | type 1 | Homo sapiens | ? | - |
? | |
| Laminin + H2O | - |
Homo sapiens | ? | - |
? | |
| additional information | may play a role in the normal turnover of the connective tissue matrix as well as in the joint destruction of chronic synovitis | Homo sapiens | ? | - |
? | |
| Procollagen + H2O | removal of the NH2-terminal propeptides | Homo sapiens | ? | - |
? | |
| Procollagen + H2O | chicken type I | Homo sapiens | ? | - |
? | |
| Proteoglycan + H2O | cartilage | Homo sapiens | More | degradation to 12.0 S fragments by the HMW form and 10.3 S fragments by the KMW enzyme | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, human, form HMW, SDS-PAGE | Homo sapiens |
| ? | x * 28000, human, form LMW, SDS-PAGE | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
15 min: 35% loss activity of enzyme form HMW, 5% loss of sctivity of enzyme form LMW | Homo sapiens |
| 100 | - |
15 min, 65% loss of activity of enzyme form HMW, 25% loss of activity of enzyme form LMW | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 7.8 | - |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | about 50% of activity maximum at pH 6.0 and 8.5 | Homo sapiens |
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