Cloned (Comment) | Organism |
---|---|
expression of neurolysin gene fron brain ligated to the C-terminal half of the alpha-agglutinin gene with a FLAG tag sequence in Saccharomyces cerevisiae at the cell surface, construction of a molecular displaying plasmid | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
neurotensin + H2O | Rattus norvegicus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | activity of the recombinant enzyme with fluorescence-quenching peptides, cleavage site and amino acid preference, overview | Rattus norvegicus | ? | - |
? | |
neurotensin + H2O | - |
Rattus norvegicus | ? | - |
? | |
[(7-methoxycoumarin-4-yl)acetyl]-APAKFFRLK(Dnp)-NH2 + H2O | best substrate | Rattus norvegicus | [(7-methoxycoumarin-4-yl)acetyl]-APAK + FFRLK(Dnp)-NH2 | - |
? | |
[(7-methoxycoumarin-4-yl)acetyl]-DEVDAPK(Dnp)-NH2 + H2O | very low activity | Rattus norvegicus | ? | - |
? | |
[(7-methoxycoumarin-4-yl)acetyl]-GKPILFFRLK(Dnp)DR-NH2 + H2O | high activity | Rattus norvegicus | [(7-methoxycoumarin-4-yl)acetyl]-GKP + ILFFRLK(Dnp)DR-NH2 | - |
? | |
[(7-methoxycoumarin-4-yl)acetyl]-GKPILFFRLK(Dnp)DR-NH2 + H2O | high activity | Rattus norvegicus | [(7-methoxycoumarin-4-yl)acetyl]-GKPIL + FFRLK(Dnp)DR-NH2 | - |
? | |
[(7-methoxycoumarin-4-yl)acetyl]-GSPAFLAK(Dnp)DR-NH2 + H2O | low activity | Rattus norvegicus | [(7-methoxycoumarin-4-yl)acetyl]-GSPA + FLAK(Dnp)DR-NH2 | - |
? | |
[(7-methoxycoumarin-4-yl)acetyl]-RPKPYANvaWMK(Dnp)-NH2 + H2O | high activity | Rattus norvegicus | [(7-methoxycoumarin-4-yl)acetyl]-RPKP + YANvaWMK(Dnp)-NH2 | - |
? | |
[(7-methoxycoumarin-4-yl)acetyl]-RPKPYANvaWMK(Dnp)-NH2 + H2O | high activity | Rattus norvegicus | [(7-methoxycoumarin-4-yl)acetyl]-RPKPY + ANvaWMK(Dnp)-NH2 | - |
? | |
[(7-methoxycoumarin-4-yl)acetyl]-RPPGFSAFK(Dnp)-OH + H2O | low activity | Rattus norvegicus | [(7-methoxycoumarin-4-yl)acetyl]-RPPGFSA + FK(Dnp)-OH | - |
? |