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Literature summary for 3.4.24.14 extracted from
- Procollagen N-endopeptidase, ADAMTS2 (2004), Handbook of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 737-740.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Dextran sulfate | increases rate of cleavage about 4fold | Bos taurus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | ADAMTS2 mutated at the furin-cleavage site separating the pro domain and the metalloprotease domain lacks enzymatic activity | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | potent inhibitor | Bos taurus |  |
| concanavalin A | potent inhibitor | Bos taurus | |
| Cu2+ | potent inhibitor | Bos taurus | |
| heparan sulfate | potent inhibitor | Bos taurus | |
| heparin | potent inhibitor | Bos taurus | |
| metal chelator | potent inhibitor | Bos taurus | |
| additional information | not inhibited by serine proteinase inhibitor | Bos taurus | |
| SDS | potent inhibitor | Bos taurus | |
| Zn2+ | potent inhibitor | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.435 | - |
procollagen I | pH 7.5, 2 mM CaCl2, 35°C | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required for activity | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 107000 | - |
chromatography | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Gallus gallus | - |
- |
- |
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
| purified 1000-16000fold to near homogeneity by different chromatographies | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bone | - |
Bos taurus | - |
| brain | - |
Bos taurus | - |
| embryo | - |
Gallus gallus | - |
| eye | - |
Bos taurus | - |
| liver | - |
Bos taurus | - |
| skin | - |
Bos taurus | - |
| tendon | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no cleavage of native procollagen III or collagen XIV, recombinant ADAMTS2 produced in 293 cells can process the N-propeptide of procollagen III in vitro | Bos taurus | ? | - |
? | |
| procollagen I + H2O | removal of the N-terminal propeptide, cleavage of the substrate in native conformation but not after heat denaturation | Bos taurus | pCCollagen I + oligopeptide | - |
? | |
| procollagen II + H2O | removal of the N-terminal propeptide, cleavage of the substrate in native conformation but not after heat denaturation | Bos taurus | pCCollagen II + oligopeptide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADAMTS2 | - |
Gallus gallus |
| ADAMTS2 | - |
Homo sapiens |
| ADAMTS2 | - |
Bos taurus |
| Procollagen I N-proteinase | - |
Gallus gallus |
| Procollagen I N-proteinase | - |
Homo sapiens |
| Procollagen I N-proteinase | - |
Bos taurus |
| procollagen I/II N-endopeptidase | - |
Gallus gallus |
| procollagen I/II N-endopeptidase | - |
Homo sapiens |
| procollagen I/II N-endopeptidase | - |
Bos taurus |
| procollagen N-protease | - |
Gallus gallus |
| procollagen N-protease | - |
Homo sapiens |
| procollagen N-protease | - |
Bos taurus |
| procollagen N-proteinase | - |
Gallus gallus |
| procollagen N-proteinase | - |
Homo sapiens |
| procollagen N-proteinase | - |
Bos taurus |
| Procollagen peptidase | - |
Gallus gallus |
| Procollagen peptidase | - |
Homo sapiens |
| Procollagen peptidase | - |
Bos taurus |
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