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Literature summary for 3.4.24.14 extracted from
- Type I procollagen N-proteinase from whole chick embryos. Cleavage of a homotrimer of pro-alpha 1(I) chains and the requirement for procollagen with a triple-helical conformation (1985), J. Biol. Chem., 260, 1120-1126.
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ stabilizes | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | 93% inhibition at 10 mM | Gallus gallus |  |
| dithiothreitol | 88% inhibition at 1 mM | Gallus gallus | |
| EDTA | 100% inhibition at 10 mM | Gallus gallus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required for maximal activity | Gallus gallus | |
| Ca2+ | 50% increase of activity between 2.5 and 20 mM | Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 320000 | - |
gel filtration | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| procollagen I + H2O | Gallus gallus | - |
? | - |
? | |
| procollagen II + H2O | Gallus gallus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| procollagen I + H2O | substrate must be in native state | Gallus gallus | pCCollagen I + oligopeptide | partially processed procollagen containing only the C-propeptides | ? | |
| procollagen I + H2O | - |
Gallus gallus | ? | - |
? | |
| procollagen II + H2O | substrate must be in native state | Gallus gallus | pCCollagen II + oligopeptide | partially processed procollagen containing only the C-propeptides | ? | |
| procollagen II + H2O | - |
Gallus gallus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36 | - |
procollagen I as substrate | Gallus gallus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | - |
inactive above, procollagen I as substrate | Gallus gallus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
90% of activity recovered after 30 min, 83% of activity recovered after 90 min | Gallus gallus |
| 48 | - |
75% of activity recovered after 30 min | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 9 | procollagen I | Gallus gallus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
inactive below, procollagen I as substrate | Gallus gallus |
| 7 | 9.3 | procollagen I as substrate, more than 50% of optimum activity | Gallus gallus |
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Release 2023.1 (January 2023)
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