Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Neisseria meningitidis |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1003-1006 | mutant lacking the confirmed self-cleavage recognition site shows only little reduction in secreted protease | Neisseria meningitidis |
DELTA1005-1006 | mutant containing modified consensus recognition sites shows only slight reduction in protease secretion | Neisseria meningitidis |
DELTA974-1002 | mutant lacking the putative self-cleavage recognition site and the intervening residues shows only little reduction in secreted protease | Neisseria meningitidis |
DELTA974-978 | mutant lacking the putative self-cleavage recognition site shows only little reduction in secreted protease | Neisseria meningitidis |
DELTA974-978/DELTA1003-1007 | double deletion mutant lacking both self-cleavage recognition sites shows only a 40% reduced secretion | Neisseria meningitidis |
additional information | a mutant in which the entire region spanning the putative (residues 974-978) and the confirmed self-cleavage site (residues 1003-1006) is replaced by a hexahistidine linker fails to produce any detectable secreted protease | Neisseria meningitidis |
P1004T/S1005E/S1006A | mutant containing modified consensus recognition sites shows only slight reduction in protease secretion | Neisseria meningitidis |
P975E/P1004E | mutant containing modified consensus recognition sites shows 30% lower secreted protease levels than wild-type | Neisseria meningitidis |
S1005E | mutant containing modified consensus recognition sites shows only slight reduction in protease secretion | Neisseria meningitidis |
S267V | this mutant fails to undergo self-cleavage and shows no enzymatic activity | Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | - |
- |
- |
Neisseria meningitidis NMB | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the unexpected self-cleavage and subsequent extracellular release of mature IgA1 protease from mutants lacking the previously defined consensus cleavage site (P-P-S-P, residues 1003-1006) and/or the putative (P-V-P-S-P, residues 1003-1006) self-cleavage site is shown | Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
IgA1 protease | - |
Neisseria meningitidis |