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Literature summary for 3.4.24.11 extracted from
- Localization of mature neprilysin in lipid rafts (2012), J. Neurosci. Res., 90, 870-877.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of N-terminally FLAG-tagged wild-type and mutant enzymes in HEK-293 cell membranes | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E403C | site-directed mutagenesis, homodimerization mutant, reduced localization in lipid rafts | Homo sapiens |
| E584V | site-directed mutagenesis, catalytically inactive mutant, negative control | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Z-Leu-Leu-Leu-H | - |
Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | localization of mature neprilysin, glycosylated in the Golgi apparatus, in membrane microdomains, i.e. lipid rafts, which are enriched in cholesterol and glycosphingolipids. Localization of the enzyme in lipid rafts is enhanced by its dimerization | Homo sapiens | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amyloid beta peptide + H2O | Homo sapiens | - |
? | - |
? | |
| additional information | Homo sapiens | the enzyme directly interacts with phosphatidylserine and cardiolipin but not with cholesterol | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P08473 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | glycosylation of the enzme in the Golgi apparatus, deglycosylation by N-glycosidase F | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amyloid beta peptide + H2O | - |
Homo sapiens | ? | - |
? | |
| additional information | the enzyme directly interacts with phosphatidylserine and cardiolipin but not with cholesterol | Homo sapiens | ? | - |
? | |
| Z-Ala-Ala-Leu-4-nitroanilide + H2O | - |
Homo sapiens | Z-Ala-Ala-Leu + 4-nitroaniline | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NEP | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | protease activities of the mature form of the enzyme do not differ between lipid rafts and nonlipid rafts. Cholesterol and other lipids regulate the localization of mature enzyme to lipid rafts, where the substrate amyloid beta peptide accumulates but does not modulate the protease activity of the enzyme | Homo sapiens |
| physiological function | neprilysin is a neutral endopeptidase, is one of the major amyloid beta-degrading enzymes in the brain | Homo sapiens |
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Release 2023.1 (January 2023)
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