Protein Variants | Comment | Organism |
---|---|---|
additional information | chemical synthesis of a full-length HTLV protease and a C-terminally truncated form encompassing residues 1-116. Constructs contain an S47C mutation for native chemical ligation and an L40I mutation that suppresses known autolysis of the protease without altering its catalytic activity. Truncation of the C-terminal tail lowers the turnover number of the viral enzyme by a factor of 2 and its catalytic efficiency by roughly tenfold | Human T-cell leukemia virus type I |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
APQVLF(NO2)VMHPL | full length enzyme, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
0.062 | - |
APQVLF(NO2)VMHPL | truncated enzyme, residues 1-116, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
0.38 | - |
APQVLPVMHP | truncated enzyme, residues 1-116, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
0.52 | - |
KTKVLVVQPK | full length enzyme, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
0.8 | - |
APQVLPVMHP | full length enzyme, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
1.5 | 2 | KTKVLVVQPK | truncated enzyme, residues 1-116, 37°C, pH 5.6 | Human T-cell leukemia virus type I |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13672 | - |
x * 13672, ESI-MS of full length enzyme | Human T-cell leukemia virus type I |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human T-cell leukemia virus type I | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
APQVLF(NO2)VMHPL + H2O | - |
Human T-cell leukemia virus type I | APQVL + F(NO2)VMHP | - |
? | |
APQVLPVMHP + H2O | - |
Human T-cell leukemia virus type I | APQVL + PVMHP | - |
? | |
KTKVLVVQPK + H2O | - |
Human T-cell leukemia virus type I | KTKVL + VVQPK | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 13672, ESI-MS of full length enzyme | Human T-cell leukemia virus type I |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
APQVLF(NO2)VMHPL | truncated enzyme, residues 1-116, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
0.161 | - |
APQVLF(NO2)VMHPL | full length enzyme, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
2.8 | - |
KTKVLVVQPK | truncated enzyme, residues 1-116, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
4.3 | - |
APQVLPVMHP | truncated enzyme, residues 1-116, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
6.7 | - |
KTKVLVVQPK | full length enzyme, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
8.3 | - |
APQVLPVMHP | full length enzyme, 37°C, pH 5.6 | Human T-cell leukemia virus type I |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
APQVLF(NO2)VMHPL | truncated enzyme, residues 1-116, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
1.14 | - |
APQVLPVMHP | truncated enzyme, residues 1-116, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
1.87 | - |
KTKVLVVQPK | truncated enzyme, residues 1-116, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
3.82 | - |
APQVLF(NO2)VMHPL | full length enzyme, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
10.4 | - |
APQVLPVMHP | full length enzyme, 37°C, pH 5.6 | Human T-cell leukemia virus type I | |
12.8 | - |
KTKVLVVQPK | full length enzyme, 37°C, pH 5.6 | Human T-cell leukemia virus type I |