Literature summary for 3.4.23.B8 extracted from

Li, M.; Laco, G.S.; Jaskolski, M.; Rozycki, J.; Alexandratos, J.; Wlodawer, A.; Gustchina, A.
Crystal structure of human T cell leukemia virus protease, a novel target for anticancer drug design (2005), Proc. Natl. Acad. Sci. USA, 102, 18332-18337.

Search for more literature for 3.4.23.B8

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant HTLV-1 PR in complex with inhibitor Ac-Ala-Pro-Gln-Val-statine-Val-Met-His-Pro, enzyme-inhibitor ratio of 1:10, 5°C, hanging drop vapor diffusion method, mxing of 0.004 ml of protein solution and of well solution, the latter containing 17% PEG 8000, 16% PEG 300, and 10 mM DTT, in 0.1 M acetate buffer, pH 5.2, X-ray diffraction structure determination and analysis at 2.6 A resolution, structure analysis and modeling, overview	Human T-cell leukemia virus type I

Crystallization (Comment)

Organism

purified recombinant HTLV-1 PR in complex with inhibitor Ac-Ala-Pro-Gln-Val-statine-Val-Met-His-Pro, enzyme-inhibitor ratio of 1:10, 5°C, hanging drop vapor diffusion method, mxing of 0.004 ml of protein solution and of well solution, the latter containing 17% PEG 8000, 16% PEG 300, and 10 mM DTT, in 0.1 M acetate buffer, pH 5.2, X-ray diffraction structure determination and analysis at 2.6 A resolution, structure analysis and modeling, overview

Human T-cell leukemia virus type I

Inhibitors

Inhibitors	Comment	Organism	Structure
Ac-Ala-Pro-Gln-Val-statine-Val-Met-His-Pro	binding mode in an extended conformation at the active site, competitive, binding-involved residues of the S2/S2'-subsite, overview	Human T-cell leukemia virus type I

Organism

Organism	UniProt	Comment	Textmining
Human T-cell leukemia virus type I	-	HTLV-1	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Ac-KDKT-(N-o-aminobenzoyl)KVL-(4-nitro)FVQPKK-NH2 + H2O	fluorogenic substrate	Human T-cell leukemia virus type I	Ac-KDKT-(N-o-aminobenzoyl)KVL + (4-nitro)FVQPKK-NH2	-	?

Subunits

Subunits	Comment	Organism
dimer	structure analysis and modeling, overview	Human T-cell leukemia virus type I

Synonyms

Synonyms	Comment	Organism
HTLV-1 PR	-	Human T-cell leukemia virus type I
human T cell leukemia virus PR	-	Human T-cell leukemia virus type I
human T cell leukemia virus protease	-	Human T-cell leukemia virus type I

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Human T-cell leukemia virus type I

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	assay at	Human T-cell leukemia virus type I

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00005	-	Ac-Ala-Pro-Gln-Val-statine-Val-Met-His-Pro	recombinant enzyme, pH 5.5, 37°C	Human T-cell leukemia virus type I