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Literature summary for 3.4.23.B6 extracted from
- The role of the S-S bridge in retroviral protease function and virion maturation (2007), J. Mol. Biol., 365, 1493-1504.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Mason-Pfizer monkey virus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.386 | - |
ATHQVYNphVRKA | 13 kDa protease in 0.05% beta-mercaptoethanol | Mason-Pfizer monkey virus |  |
| 0.42 | - |
ATHQVYNphVRKA | 13 kDa protease in 1% beta-mercaptoethanol | Mason-Pfizer monkey virus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | of host cell | Mason-Pfizer monkey virus | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13000 | - |
SDS-PAGE, the protease is excised autocatalytically from Gag polyprotein precursors in a 17 kDa form that undergoes further C-terminal self-processing to yield the 13 kDa protease. | Mason-Pfizer monkey virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mason-Pfizer monkey virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| dialysis and batch chromatography on QAE-Sephadex A25 | Mason-Pfizer monkey virus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | transfection of COS-1 cells with the proviral construct | Mason-Pfizer monkey virus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATHQVYNphVRKA + H2O | - |
Mason-Pfizer monkey virus | hydrolyzed peptid | the cysteine residues in M-PMV protease form an intramolecular disulfide bridge and that increases the proteolytic activity significantly | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | The active form of the protease is a homodimer with a single active site. The two subunits are stabilized by hydrogen bonding interactions within the fourstranded antiparallel beta-sheet. Substrate binding stabilizes the dimer. | Mason-Pfizer monkey virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| M-PMV protease | - |
Mason-Pfizer monkey virus |
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Release 2023.1 (January 2023)
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