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Literature summary for 3.4.23.B6 extracted from
- Cloning, bacterial expression, and characterization of the Mason-Pfizer monkey virus proteinase (1995), J. Biol. Chem., 270, 15053-15058.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and expression of the 3'-region of the Mason-Pfizer monkey virus pro gene in Escherichia coli | Mason-Pfizer monkey virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PCV-phenylstatine-AMT | - |
Mason-Pfizer monkey virus |  |
| PMV-phenylstatine-VRP | - |
Mason-Pfizer monkey virus | |
| PPAV-cysteinestatine-AMTM | - |
Mason-Pfizer monkey virus | |
| PPCV-phenylstatine-AMTM | - |
Mason-Pfizer monkey virus | |
| PPYV-phenylstatine-AMTM | - |
Mason-Pfizer monkey virus | |
| PYV-phenylstatine-AMT | - |
Mason-Pfizer monkey virus | |
| RPMV-phenylstatine-VMP | - |
Mason-Pfizer monkey virus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0034 | - |
ATPQVYF(NO2)VRKA | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 0.038 | - |
SSDIYWVQPI | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 0.298 | - |
PKQAYGAVFV | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 0.417 | - |
APTVMAVVNP | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 0.517 | - |
PKDIFPVTET | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mason-Pfizer monkey virus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the 26000 Da precursor undergoes rapid self-processing both in Escherichia coli and in vitro at the NH2 terminus, yielding a proteolytically active 17000 Da protein, p17. This initial cleavage is followed in vitro by a much slower self-processing that leads to emergence of proteolytically active P12 and a COOH-terminal cleavage product, p5 | Mason-Pfizer monkey virus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mason-Pfizer monkey virus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 5.5, the 17000 Da species is self-processed slowly into the 12000 Da form and the 5000 Da peptide | Mason-Pfizer monkey virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| APTVMAVVNP + H2O | - |
Mason-Pfizer monkey virus | ? | - |
? | |
| ATPQVYF(NO2)VRKA + H2O | - |
Mason-Pfizer monkey virus | ATPQVY + F(NO2)VRKA | - |
? | |
| GPEPPAVSLAMTDHK + H2O | - |
Mason-Pfizer monkey virus | GPEPPAVS + LAMTDHK | - |
? | |
| IMMCSPNDI + H2O | - |
Mason-Pfizer monkey virus | IMMCS + PNDI | - |
? | |
| PKDIFPVTET + H2O | - |
Mason-Pfizer monkey virus | PKDIF + PVTET | - |
? | |
| PKQAYGAVFV + H2O | - |
Mason-Pfizer monkey virus | PKQAY + GAVFV | - |
? | |
| PKSIFPVTET + H2O | - |
Mason-Pfizer monkey virus | PKSIF + PVTET | - |
? | |
| QGTVSFNFPQITLVWOK + H2O | - |
Mason-Pfizer monkey virus | QGTVSFNF + PQITLVWOK | - |
? | |
| SSDIYWVQPI + H2O | - |
Mason-Pfizer monkey virus | SSDIY + WVQPI | - |
? | |
| TGPPVVAMPVVIKTEG + H2O | - |
Mason-Pfizer monkey virus | TGPPVVAM + PVVIKTEG | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.56 | - |
SSDIYWVQPI | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 4.65 | - |
APTVMAVVNP | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 7 | - |
ATPQVYF(NO2)VRKA | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 7.6 | - |
PKDIFPVTET | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 7.7 | - |
PKQAYGAVFV | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Mason-Pfizer monkey virus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000003 | - |
PYV-phenylstatine-AMT | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 0.00003 | - |
PPYV-phenylstatine-AMTM | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 0.00005 | - |
RPMV-phenylstatine-VMP | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 0.00006 | - |
PCV-phenylstatine-AMT | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 0.00028 | - |
PMV-phenylstatine-VRP | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 0.0031 | - |
PPCV-phenylstatine-AMTM | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
| 0.013 | - |
PPAV-cysteinestatine-AMTM | pH 5.3, 37°C | Mason-Pfizer monkey virus | |
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