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Literature summary for 3.4.23.B6 extracted from

  • Zabransky, A.; Andreansky, M.; Hruskova-Heidingsfeldova, O.; Havlicek, V.; Hunter, E.; Ruml, T.; Pichova, I.
    Three active forms of aspartic proteinase from Mason-Pfizer monkey virus (1998), Virology, 245, 250-256.
    View publication on PubMed
Search for more literature for 3.4.23.B6

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12250
-
 three active forms of a retroviral enzyme are determined by electrospray ionization mass spectroscopy: 12246 Da, 12956 Da and 16965 Da Mason-Pfizer monkey virus
12960
-
 three active forms of a retroviral enzyme are determined by electrospray ionization mass spectroscop: 12246 Da, 12956 Da and 16965 Da Mason-Pfizer monkey virus
16970
-
 three active forms of a retroviral enzyme are determined by electrospray ionization mass spectroscop: 12246 Da, 12956 Da and 16965 Da Mason-Pfizer monkey virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mason-Pfizer monkey virus the individual forms of M-PMV proteinase may be responsible for cleavage at different locations in the virion or at different stages of maturation ?
-
 ?
additional information Mason-Pfizer monkey virus the enzyme is released by the autocatalytic cleavage of Gag-Pro and Gag-Pro-POl polypeptide precursors. The enzyme catalyzes the processing of viral precursors to yield the structural proteins and enzymes of the virion ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mason-Pfizer monkey virus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification processing of the 26000 Da precursor results in the first product of autocatalytic cleavage of 17000 Da, that can be processed further into truncated but still proteolytically active enzymes of 13000 Da and 120000 Da Mason-Pfizer monkey virus

Purification (Commentary)

Purification (Comment) Organism
-
 Mason-Pfizer monkey virus

Storage Stability

Storage Stability Organism
4°C, 13000 Da enzyme form is stable at pH 6.4 and at salt concentrations lower than 0.1 M Mason-Pfizer monkey virus
the mixture of 17000 Da and 13000 Da enzyme, purified on QAE-Sephadex, is stable in Tris-HCl buffer, pH 7.5, at 4°C for at least 1 year Mason-Pfizer monkey virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the individual forms of M-PMV proteinase may be responsible for cleavage at different locations in the virion or at different stages of maturation Mason-Pfizer monkey virus ?
-
 ?
additional information the enzyme is released by the autocatalytic cleavage of Gag-Pro and Gag-Pro-POl polypeptide precursors. The enzyme catalyzes the processing of viral precursors to yield the structural proteins and enzymes of the virion Mason-Pfizer monkey virus ?
-
 ?

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
 the 13000 Da enzyme is stable Mason-Pfizer monkey virus