Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, wild-type and mutant enzymes are expressed in fusion with the Schistosoma japonicum glutathione S-transferase | Moloney murine leukemia virus |
Protein Variants | Comment | Organism |
---|---|---|
A57I | mutation has a strong influence on substrate specificity | Moloney murine leukemia virus |
C88T | mutant enzyme shows an increased preference for hydrophobic amino acids at P4 and P2 position in a series of VSQNYPIVQ analogs | Moloney murine leukemia virus |
E15R | mutant enzyme shows similar kinetic parameters to that of the mutant enzyme | Moloney murine leukemia virus |
G60F | mutant enzyme shows an increased preference for hydrophobic amino acids at P4 and P2 position in a series of VSQNYPIVQ analogs | Moloney murine leukemia virus |
H37D | mutation has a strong influence on substrate specificity | Moloney murine leukemia virus |
L92I | mutant enzyme shows an increased preference for hydrophobic amino acids at P4 and P2 position in a series of VSQNYPIVQ analogs | Moloney murine leukemia virus |
L92I | mutation has a strong influence on substrate specificity | Moloney murine leukemia virus |
V39I | mutant enzyme shows similar kinetic parameters to that of the mutant enzyme | Moloney murine leukemia virus |
V39I | mutation has a strong influence on substrate specificity | Moloney murine leukemia virus |
V39I/V54I | mutant enzyme shows similar kinetic parameters to that of the mutant enzyme | Moloney murine leukemia virus |
V54I | mutant enzyme shows similar kinetic parameters to that of the mutant enzyme | Moloney murine leukemia virus |
V54I | mutant enzyme shows an increased preference for hydrophobic amino acids at P4 and P2 position in a series of VSQNYPIVQ analogs | Moloney murine leukemia virus |
W53I | mutant enzyme shows an increased preference for hydrophobic amino acids at P4 and P2 position in a series of VSQNYPIVQ analogs | Moloney murine leukemia virus |
W53I/Q55G | mutant enzyme does not cleave the wild-type substrates: VSQNYPIVQ, VIQNYPIVQ and VSQNYPLVQ | Moloney murine leukemia virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
VSQNYPLVQ | pH 6.0, 37°C, mutant enzyme V54I | Moloney murine leukemia virus | |
0.019 | - |
VSQNYPLVQ | pH 6.0, 37°C, wild-type enzyme | Moloney murine leukemia virus | |
0.029 | - |
VSQNYPLVQ | pH 6.0, 37°C, mutant enzyme A57I | Moloney murine leukemia virus | |
0.036 | - |
VIQNYPIVQ | pH 6.0, 37°C, mutant enzyme A57I | Moloney murine leukemia virus | |
0.039 | - |
VIQNYPIVQ | pH 6.0, 37°C, wild-type enzyme | Moloney murine leukemia virus | |
0.041 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme H37D | Moloney murine leukemia virus | |
0.049 | - |
VSQNYPLVQ | pH 6.0, 37°C, mutant enzyme V39I | Moloney murine leukemia virus | |
0.052 | - |
VSQNYPLVQ | pH 6.0, 37°C, mutant enzyme V39I/V54I and V39I/A57I | Moloney murine leukemia virus | |
0.061 | - |
VIQNYPIVQ | pH 6.0, 37°C, mutant enzyme V39I/A57I | Moloney murine leukemia virus | |
0.074 | - |
VSQNYPLVQ | pH 6.0, 37°C, mutant enzyme L92I | Moloney murine leukemia virus | |
0.134 | - |
VIQNYPIVQ | pH 6.0, 37°C, mutant enzyme V39I | Moloney murine leukemia virus | |
0.174 | - |
VIQNYPIVQ | pH 6.0, 37°C, mutant enzyme L92I | Moloney murine leukemia virus | |
0.234 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme V54I | Moloney murine leukemia virus | |
0.261 | - |
VSQNYPIVQ | pH 6.0, 37°C, wild-type enzyme | Moloney murine leukemia virus | |
0.265 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme E15R | Moloney murine leukemia virus | |
0.268 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme V39I | Moloney murine leukemia virus | |
0.287 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme V39I/V54I | Moloney murine leukemia virus | |
0.29 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme Y63V | Moloney murine leukemia virus | |
0.356 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme A57I | Moloney murine leukemia virus | |
0.456 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme V39I/A57I | Moloney murine leukemia virus | |
1.238 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme L92I | Moloney murine leukemia virus | |
2.046 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme W53I | Moloney murine leukemia virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moloney murine leukemia virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
VIQNYPIVQ + H2O | - |
Moloney murine leukemia virus | VIQNY + PIVQ | - |
? | |
VSQNYPIVQ + H2O | - |
Moloney murine leukemia virus | VSQNY + PIVQ | - |
? | |
VSQNYPLVQ + H2O | - |
Moloney murine leukemia virus | VSQNY + PLVQ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
VSQNYPLVQ | pH 6.0, 37°C, mutant enzyme V39I/A57I | Moloney murine leukemia virus | |
0.012 | - |
VIQNYPIVQ | pH 6.0, 37°C, mutant enzyme H37D | Moloney murine leukemia virus | |
0.023 | - |
VSQNYPLVQ | pH 6.0, 37°C, mutant enzyme H37D | Moloney murine leukemia virus | |
0.0442 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme L92I | Moloney murine leukemia virus | |
0.062 | - |
VSQNYPLVQ | pH 6.0, 37°C, mutant enzyme A57I | Moloney murine leukemia virus | |
0.065 | - |
VIQNYPIVQ | pH 6.0, 37°C, mutant enzyme V39I/A57I | Moloney murine leukemia virus | |
0.072 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme V39I/A57I | Moloney murine leukemia virus | |
0.075 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme H37D | Moloney murine leukemia virus | |
0.11 | - |
VSQNYPLVQ | pH 6.0, 37°C, mutant enzyme V39I/V54I | Moloney murine leukemia virus | |
0.12 | - |
VIQNYPIVQ | pH 6.0, 37°C, mutant enzyme L92I | Moloney murine leukemia virus | |
0.2 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme Y63V | Moloney murine leukemia virus | |
0.2 | - |
VSQNYPLVQ | pH 6.0, 37°C, mutant enzyme V39I | Moloney murine leukemia virus | |
0.22 | - |
VIQNYPIVQ | pH 6.0, 37°C, mutant enzyme V39I | Moloney murine leukemia virus | |
0.23 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme A57I | Moloney murine leukemia virus | |
0.24 | - |
VSQNYPLVQ | pH 6.0, 37°C, wild-type enzyme | Moloney murine leukemia virus | |
0.3 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzymes E15R and W53I | Moloney murine leukemia virus | |
0.34 | - |
VSQNYPLVQ | pH 6.0, 37°C, mutant enzyme L92I | Moloney murine leukemia virus | |
0.35 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme V54I | Moloney murine leukemia virus | |
0.4 | - |
VIQNYPIVQ | pH 6.0, 37°C, mutant enzyme A57I | Moloney murine leukemia virus | |
0.46 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme V39I/V54I | Moloney murine leukemia virus | |
0.48 | - |
VIQNYPIVQ | pH 6.0, 37°C, wild-type enzyme | Moloney murine leukemia virus | |
0.55 | - |
VSQNYPIVQ | pH 6.0, 37°C, wild-type enzyme | Moloney murine leukemia virus | |
0.74 | - |
VSQNYPIVQ | pH 6.0, 37°C, mutant enzyme V39I | Moloney murine leukemia virus | |
1.9 | - |
VSQNYPLVQ | pH 6.0, 37°C, mutant enzyme V54I | Moloney murine leukemia virus |