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Literature summary for 3.4.23.B4 extracted from

  • Lin, Y.C.; Beck, Z.; Lee, T.; Le, V.D.; Morris, G.M.; Olson, A.J.; Wong, C.H.; Elder, J.H.
    Alteration of substrate and inhibitor specificity of feline immunodeficiency virus protease (2000), J. Virol., 74, 4710-4720.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.B4

Protein Variants

Protein Variants Comment Organism
I35D mutant enzyme shows no activity against either FIV or HIV substrates feline immunodeficiency virus
I35D/I37V mutant enzyme shows no activity against either FIV or HIV substrates feline immunodeficiency virus
I35D/I37V/L97T/I98P/Q99V/P100N/L101I mutant enzyme shows no activity against either FIV or HIV substrates feline immunodeficiency virus
I35D/I37V/Q54K/N55M/M56I/I57G/V59I mutant enzyme shows no activity against either FIV or HIV substrates feline immunodeficiency virus
I35D/I37V/Q54K/N55M/M56I/I57G/V59I/L97T/I98P/Q99V/ mutant enzyme shows no activity against either FIV or HIV substrates feline immunodeficiency virus
I35D/I57G mutant enzyme shows no activity against either FIV or HIV substrates feline immunodeficiency virus
I35D/M56I mutant enzyme shows no activity against either FIV or HIV substrates feline immunodeficiency virus
I35D/Q54K mutant enzyme shows no activity against either FIV or HIV substrates feline immunodeficiency virus
I35D/V59I mutant enzyme shows no activity against either FIV or HIV substrates feline immunodeficiency virus
I37V mutant enzyme with full activity. Significant increase in activity against the HIV-I reverse transcriptase/integrase and P2/nucleocapsid junction peptides compared with wild-type enzyme. Increase in activity against two rapidly cleaved peptides selected by cleavage of phage display library with HIV-I protease. Mutant enzyme compared with wild-type enzyme shows inhibitor specificity more similar to that of the HIV-1 protease feline immunodeficiency virus
I57G mutant enzyme shows no activity against either FIV or HIV substrates feline immunodeficiency virus
I98P mutant enzyme with dramatically reduced activity feline immunodeficiency virus
L101I mutant enzyme with dramatically reduced activity feline immunodeficiency virus
M56I mutant enzyme with full activity. Mutant enzyme compared with wild-type enzyme shows inhibitor specificity more similar to that of the HIV-1 protease feline immunodeficiency virus
N55M mutant enzyme with full activity. Significant increase in activity against the HIV-I reverse transcriptase/integrase and P2/nucleocapsid junction peptides compared with wild-type enzyme. Mutant enzyme compared with wild-type enzyme shows inhibitor specificity more similar to that of the HIV-1 protease feline immunodeficiency virus
Q64K mutant enzyme with dramatically reduced activity feline immunodeficiency virus
Q99V mutant enzyme with full activit. Significant increase in activity against the HIV-I reverse transcriptase/integrase and P2/nucleocapsid junction peptides compared with wild-type enzyme. Increase in activity against two rapidly cleaved peptides selected by cleavage of phage display library with HIV-I protease. Mutant enzyme compared with wild-type enzyme shows inhibitor specificity more similar to that of the HIV-1 protease feline immunodeficiency virus
V59I mutant enzyme with full activity. Significant increase in activity against the HIV-I reverse transcriptase/integrase and P2/nucleocapsid junction peptides compared with wild-type enzym. Increase in activity against two rapidly cleaved peptides selected by cleavage of phage display library with HIV-I protease. Mutant enzyme compared with wild-type enzyme shows inhibitor specificity more similar to that of the HIV-1 protease feline immunodeficiency virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0056
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I37V/V59I feline immunodeficiency virus
0.01
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I37V feline immunodeficiency virus
0.0114
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme V59I feline immunodeficiency virus
0.0156
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme N55M feline immunodeficiency virus
0.0159
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I37V/N55M feline immunodeficiency virus
0.018
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme Q99V feline immunodeficiency virus
0.0193
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme L97T/I98P/Q99V/P100N/L101I feline immunodeficiency virus
0.0205
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I37V/M56I feline immunodeficiency virus
0.0244
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme L101I feline immunodeficiency virus
0.0256
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, wild-type enzyme feline immunodeficiency virus
0.0278
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme M56I feline immunodeficiency virus
0.0351
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I98P feline immunodeficiency virus
0.0372
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I37V/Q54K feline immunodeficiency virus
0.156
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme Q54K feline immunodeficiency virus
0.271
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I57G/G62F feline immunodeficiency virus
0.462
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme Q99V feline immunodeficiency virus
0.542
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme I37V feline immunodeficiency virus
0.582
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme N55M feline immunodeficiency virus
0.739
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme I37V/N55M feline immunodeficiency virus
0.832
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme I37V/V59I feline immunodeficiency virus
1.183
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme V59I feline immunodeficiency virus
1.4
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, wild-type enzyme feline immunodeficiency virus
1.845
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme M56I feline immunodeficiency virus

Organism

Organism UniProt Comment Textmining
feline immunodeficiency virus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly + H2O
-
 feline immunodeficiency virus Ala-Leu-Thr-Lys-Val-Gln + Val-Val-Gln-Ser-Lys-Gly
-
 ?
Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly + H2O
-
 feline immunodeficiency virus Ile-Arg-Lys-Ile-Leu + Phe-Leu-Asp-Gly
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0015
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I57G/G62F feline immunodeficiency virus
0.002
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme L101I feline immunodeficiency virus
0.0035
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme L97T/I98P/Q99V/P100N/L101I feline immunodeficiency virus
0.00367
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme Q54K feline immunodeficiency virus
0.00533
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I37V/Q54K feline immunodeficiency virus
0.00617
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I37V/V59I feline immunodeficiency virus
0.00833
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme V59I feline immunodeficiency virus
0.00983
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I37V feline immunodeficiency virus
0.0102
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I37V/N55M feline immunodeficiency virus
0.0113
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I98P feline immunodeficiency virus
0.0123
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme N55M feline immunodeficiency virus
0.0142
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme M56I feline immunodeficiency virus
0.0165
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme Q99V feline immunodeficiency virus
0.0173
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, wild-type enzyme feline immunodeficiency virus
0.0223
-
 Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, mutant enzyme I37V/V59I feline immunodeficiency virus
0.945
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme M56I feline immunodeficiency virus
1.81
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, wild-type enzyme feline immunodeficiency virus
2.27
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme I37V/V59I feline immunodeficiency virus
2.51
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme V59I feline immunodeficiency virus
2.65
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme Q99V feline immunodeficiency virus
2.69
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme N55M feline immunodeficiency virus
3.56
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme I37V feline immunodeficiency virus
4
-
 Ile-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly pH 5.25, mutant enzyme I37V/N55M feline immunodeficiency virus