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Literature summary for 3.4.23.B4 extracted from

  • Lin, Y.C.; Beck, Z.; Morris, G.M.; Olson, A.J.; Elder, J.H.
    Structural basis for distinctions between substrate and inhibitor specificities for feline immunodeficiency virus and human immunodeficiency virus proteases (2003), J. Virol., 77, 6589-6600.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information multiple-substitution mutants are constructed by replacing the residues in and around the active site of the enzyme with structurally equivalent residues of HIV-1 protease. The findings indicate that maintainance of both substrate and inhibitor specificity as a function of interactions between residues both inside and outside the active site feline immunodeficiency virus

Inhibitors

Inhibitors Comment Organism Structure
additional information KI-values for multiple-substitution mutants constructed by replacing the residues in and around the active site of the enzyme with structurally equivalent residues of HIV-1 protease. Non of the potent HIV-1 inhibitors - saquinavir, ritonavir, nelfinavir and JE-2147 - are good inhibitors for the wild-type enzyme feline immunodeficiency virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-values for multiple-substitution mutants constructed by replacing the residues in and around the active site of the enzyme with structurally equivalent residues of HIV-1 protease feline immunodeficiency virus
0.0204
-
Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, wild-type enzyme feline immunodeficiency virus

Organism

Organism UniProt Comment Textmining
feline immunodeficiency virus P16088
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Leu-Thr-(4-aminobenzoic acid)-Lys-Val-Gln-p-NO2-Phe-Val-Gln-Ser-Lys-Gly + H2O
-
feline immunodeficiency virus Ala-Leu-Thr-(4-aminobenzoic acid)-Lys-Val-Gln + p-NO2-Phe-Val-Gln-Ser-Lys-Gly
-
?
Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly + H2O
-
feline immunodeficiency virus Ala-Leu-Thr-Lys-Val-Gln + Val-Val-Gln-Ser-Lys-Gly
-
?
Thr-Ile-Met-Met-Gln-Arg + H2O
-
feline immunodeficiency virus Thr-Ile-Met + Met-Gln-Arg
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.01
-
Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly pH 5.25, wild-type enzyme feline immunodeficiency virus