Protein Variants | Comment | Organism |
---|---|---|
additional information | multiple-substitution mutants are constructed by replacing the residues in and around the active site of the enzyme with structurally equivalent residues of HIV-1 protease. The findings indicate that maintainance of both substrate and inhibitor specificity as a function of interactions between residues both inside and outside the active site | feline immunodeficiency virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | KI-values for multiple-substitution mutants constructed by replacing the residues in and around the active site of the enzyme with structurally equivalent residues of HIV-1 protease. Non of the potent HIV-1 inhibitors - saquinavir, ritonavir, nelfinavir and JE-2147 - are good inhibitors for the wild-type enzyme | feline immunodeficiency virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values for multiple-substitution mutants constructed by replacing the residues in and around the active site of the enzyme with structurally equivalent residues of HIV-1 protease | feline immunodeficiency virus | |
0.0204 | - |
Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly | pH 5.25, wild-type enzyme | feline immunodeficiency virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
feline immunodeficiency virus | P16088 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Leu-Thr-(4-aminobenzoic acid)-Lys-Val-Gln-p-NO2-Phe-Val-Gln-Ser-Lys-Gly + H2O | - |
feline immunodeficiency virus | Ala-Leu-Thr-(4-aminobenzoic acid)-Lys-Val-Gln + p-NO2-Phe-Val-Gln-Ser-Lys-Gly | - |
? | |
Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly + H2O | - |
feline immunodeficiency virus | Ala-Leu-Thr-Lys-Val-Gln + Val-Val-Gln-Ser-Lys-Gly | - |
? | |
Thr-Ile-Met-Met-Gln-Arg + H2O | - |
feline immunodeficiency virus | Thr-Ile-Met + Met-Gln-Arg | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.01 | - |
Ala-Leu-Thr-Lys-Val-Gln-Val-Val-Gln-Ser-Lys-Gly | pH 5.25, wild-type enzyme | feline immunodeficiency virus |