Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | equine infectious anemia virus |
Protein Variants | Comment | Organism |
---|---|---|
I54G | mutant enzyme is only marginally different from wild-type enzyme in its susceptibility to most of the inhibitors of the HIV-1/HIV-2 proteinases. LP-149 shows a ninefold reduction in potency against the mutant enzyme. Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn is cleaved less efficiently than by the wild-type enzyme. The ability to hydrolyze Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu-p-nitrophenylalanine-Ala-Lys-Ala-Leu is lowered by at least 100fold compared to wild-type enzyme | equine infectious anemia virus |
T30D | mutant enzyme is only marginally different from wild-type enzyme in its susceptibility to most of the inhibitors of the HIV-1/HIV-2 proteinases, except that HBY-793 has a potency that is reduced by ten-fold | equine infectious anemia virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ABT-538 | - |
equine infectious anemia virus | |
DMP-323 | - |
equine infectious anemia virus | |
HBY-793 | - |
equine infectious anemia virus | |
LP-149 | - |
equine infectious anemia virus | |
Ro32-2175 | - |
equine infectious anemia virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
Lys-Ala-Arg-Val-Asn-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.01 | - |
Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.01 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.01 | - |
Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu-p-nitrophenylalanine-Ala-Lys-Ala-Leu | pH 4.7, wild-type enzyme | equine infectious anemia virus | |
0.01 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
0.02 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme and mutant enzyme I54G | equine infectious anemia virus | |
0.02 | - |
Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme T30D | equine infectious anemia virus | |
0.025 | - |
Lys-Ala-Arg-Val-Leu-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.03 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme T30D | equine infectious anemia virus | |
0.035 | - |
Lys-Ala-Arg-Val-Nal-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.035 | - |
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.04 | - |
Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.04 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.04 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme T30D | equine infectious anemia virus | |
0.045 | - |
Lys-Ala-Arg-Val-Nal-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
0.05 | - |
Lys-Ala-Arg-Val-Trp-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.05 | - |
Lys-Ala-Arg-Val-Leu-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | pH 4.7, 25°C, mutant enzyme T30D | equine infectious anemia virus | |
0.06 | - |
Lys-Ala-Arg-Val-Tyr-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.06 | - |
Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme T30D | equine infectious anemia virus | |
0.07 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | pH 4.7, 25°C, mutant enzyme T30D | equine infectious anemia virus | |
0.07 | - |
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | pH 4.7, 25°C, mutant enzyme T30D | equine infectious anemia virus | |
0.075 | - |
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.075 | - |
Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
0.1 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Gln-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.1 | - |
Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn | pH 4.7, wild-type enzyme and mutant enzyme T30D | equine infectious anemia virus | |
0.105 | - |
Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
0.165 | - |
Lys-Ala-Arg-Val-Tyr-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
0.205 | - |
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
0.21 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
0.55 | - |
Lys-Ala-Arg-Val-Trp-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
x * 12000, SDS-PAGE | equine infectious anemia virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
equine infectious anemia virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
equine infectious anemia virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu-p-nitrophenylalanine-Ala-Lys-Ala-Leu + H2O | - |
equine infectious anemia virus | Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu + p-nitrophenylalanine-Ala-Lys-Ala-Leu | - |
? | |
Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 + H2O | - |
equine infectious anemia virus | Lys-Ala-Arg-Ile-2-aminohexanoic acid + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | - |
? | |
Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 + H2O | - |
equine infectious anemia virus | Lys-Ala-Arg-Leu-2-aminohexanoic acid + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | - |
? | |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 + H2O | - |
equine infectious anemia virus | Lys-Ala-Arg-Val-2-aminohexanoic acid + p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 | - |
? | |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Gln-Ala-2-aminohexanoic acid-NH2 + H2O | - |
equine infectious anemia virus | Lys-Ala-Arg-Val-2-aminohexanoic acid + p-nitrophenylalanine-Gln-Ala-2-aminohexanoic acid-NH2 | - |
? | |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 + H2O | - |
equine infectious anemia virus | Lys-Ala-Arg-Val-2-aminohexanoic acid + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | - |
? | |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 + H2O | - |
equine infectious anemia virus | Lys-Ala-Arg-Val-2-aminohexanoic acid + p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 | - |
? | |
Lys-Ala-Arg-Val-Asn-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O | - |
equine infectious anemia virus | Lys-Ala-Arg-Val-Asn + p-nitrophenylalanine-Glu-Ala-Gly-NH2 | - |
? | |
Lys-Ala-Arg-Val-L-2-naphthylalanine-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O | - |
equine infectious anemia virus | Lys-Ala-Arg-Val-L-2-naphthylalanine + p-nitrophenylalanine-Glu-Ala-Gly-NH2 | - |
? | |
Lys-Ala-Arg-Val-Leu-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 + H2O | - |
equine infectious anemia virus | Lys-Ala-Arg-Val-Leu + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | - |
? | |
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 + H2O | - |
equine infectious anemia virus | Lys-Ala-Arg-Val-Phe + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | - |
? | |
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O | - |
equine infectious anemia virus | Lys-Ala-Arg-Val-Phe + p-nitrophenylalanine-Glu-Ala-Gly-NH2 | - |
? | |
Lys-Ala-Arg-Val-Trp-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O | - |
equine infectious anemia virus | Lys-Ala-Arg-Val-Trp + p-nitrophenylalanine-Glu-Ala-Gly-NH2 | - |
? | |
Lys-Ala-Arg-Val-Tyr-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O | - |
equine infectious anemia virus | Lys-Ala-Arg-Val-Tyr + p-nitrophenylalanine-Glu-Ala-Gly-NH2 | - |
? | |
Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn + H2O | - |
equine infectious anemia virus | Pro-Ser-Glu-Glu-Tyr + Pro-Ile-2-aminohexanoic acid-Ile-Asn | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 12000, SDS-PAGE | equine infectious anemia virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Lys-Ala-Arg-Val-Asn-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.2 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
0.3 | - |
Lys-Ala-Arg-Val-Tyr-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
0.5 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.5 | - |
Lys-Ala-Arg-Val-Trp-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
0.6 | - |
Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
0.8 | - |
Lys-Ala-Arg-Val-Trp-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
0.8 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
0.8 | - |
Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn | pH 4.7, 25°C, mutant enzyme T30D | equine infectious anemia virus | |
1 | - |
Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
1 | - |
Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
1 | - |
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
1.3 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
1.3 | - |
Lys-Ala-Arg-Val-Leu-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
1.3 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
1.4 | - |
Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
1.5 | - |
Lys-Ala-Arg-Val-Nal-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, mutant enzyme I54G | equine infectious anemia virus | |
1.5 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme T30D | equine infectious anemia virus | |
1.6 | - |
Lys-Ala-Arg-Val-Nal-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
1.7 | - |
Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
1.9 | - |
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
1.9 | - |
Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu-p-nitrophenylalanine-Ala-Lys-Ala-Leu | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
2.2 | - |
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
2.5 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
2.6 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Gln-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
3 | - |
Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu-p-nitrophenylalanine-Ala-Lys-Ala-Leu | pH 4.7, 25°C, mutant enzyme T30D | equine infectious anemia virus | |
3.1 | - |
Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
4.2 | - |
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
4.2 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme T30D | equine infectious anemia virus | |
6.3 | - |
Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme T30D | equine infectious anemia virus | |
7.2 | - |
Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 | pH 4.7, 25°C, mutant enzyme T30D | equine infectious anemia virus | |
8.2 | - |
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
12.5 | - |
Lys-Ala-Arg-Val-Leu-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus | |
12.5 | - |
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 | pH 4.7, 25°C, wild-type enzyme | equine infectious anemia virus |