Literature summary for 3.4.23.B20 extracted from

Fritsche, E.; Paschos, A.; Beisel, H.G.; Bock, A.; Huber, R.
Crystal structure of the hydrogenase maturating endopeptidase HYBD from Escherichia coli (1999), J. Mol. Biol., 288, 989-998 .

Search for more literature for 3.4.23.B20

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21 (DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of HYBD are grown at 20°C by vapor-diffusion using the hanging drop method. Crystal structure of HYBD at 2.2 A resolution. It consists of a twisted five-stranded beta-sheetsurrounded by four and three helices, respectively, on each side. A cadmium ion from the crystallization buffer binds to the proposed nickel-binding site and is penta-coordinated by Glu16, Asp62, His93, and a water molecule in a pseudo-tetragonal arrangement. HYBD is topologically related to members of the metzincins superfamily of zinc endoproteinases, sharing the central beta-sheet and three helices. In contrast to the metzincins, the metal-binding site of HYBD is localized at the C-terminal end of the beta-sheet	Escherichia coli

Crystallization (Comment)

Organism

crystals of HYBD are grown at 20°C by vapor-diffusion using the hanging drop method. Crystal structure of HYBD at 2.2 A resolution. It consists of a twisted five-stranded beta-sheetsurrounded by four and three helices, respectively, on each side. A cadmium ion from the crystallization buffer binds to the proposed nickel-binding site and is penta-coordinated by Glu16, Asp62, His93, and a water molecule in a pseudo-tetragonal arrangement. HYBD is topologically related to members of the metzincins superfamily of zinc endoproteinases, sharing the central beta-sheet and three helices. In contrast to the metzincins, the metal-binding site of HYBD is localized at the C-terminal end of the beta-sheet

Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	a cadmium ion from the crystallization buffer binds to the proposed nickel-binding site and is penta-coordinated by Glu16, Asp62, His93, and a water molecule in a pseudo-tetragonal arrangement. HYBD is topologically related to members of the metzincins superfamily of zinc endoproteinases, sharing the central beta-sheet and three helices. In contrast to the metzincins, the metal-binding site of HYBD is localized at the C-terminal end of the beta-sheet	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
precursor of the large subunit of hydrogenase 2 + H2O	Escherichia coli	the maturation of [NiFe] hydrogenases includes formation of the nickel metallocenter, proteolytic processing of the metal center carrying large subunit, and its assembling with other hydrogenase subunits. The hydrogenase maturating enzyme HYBD specifically cleaves off a 15 amino acid peptide from the C terminus of the precursor of the large subunit of hydrogenase 2 (UniProt: P0ACE0)	large subunit of hydrogenase 2 + propeptide of hydrogenase 2	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P37182	MC4100	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
precursor of the large subunit of hydrogenase 2 + H2O	the maturation of [NiFe] hydrogenases includes formation of the nickel metallocenter, proteolytic processing of the metal center carrying large subunit, and its assembling with other hydrogenase subunits. The hydrogenase maturating enzyme HYBD specifically cleaves off a 15 amino acid peptide from the C terminus of the precursor of the large subunit of hydrogenase 2 (UniProt: P0ACE0)	Escherichia coli	large subunit of hydrogenase 2 + propeptide of hydrogenase 2	-	?
precursor of the large subunit of hydrogenase 2 + H2O	specifically cleaves off a 15 amino acid peptide from the C terminus of the precursor of the large subunit of hydrogenase 2 (UniProt: P0ACE0)	Escherichia coli	large subunit of hydrogenase 2 + propeptide of hydrogenase 2	-	?

Synonyms

Synonyms	Comment	Organism
endopeptidase of the hydrogenase 2 operon	-	Escherichia coli
HYBD	-	Escherichia coli
hydrogenase maturating endopeptidase HYBD	-	Escherichia coli