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Literature summary for 3.4.23.B2 extracted from

  • Grant, S.K.; Deckman, I.C.; Minnich, M.D.; Culp, J.; Franklin, S.; Dreyer, G.B.; Tomaszek, T.A., Jr.; Debouck, C.; Meek, T.D.
    Purification and biochemical characterization of recombinant simian immunodeficiency virus protease and comparison to human immunodeficiency virus type 1 protease (1991), Biochemistry, 30, 8424-8434.
    View publication on PubMed
Search for more literature for 3.4.23.B2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli with a recombinant expression system Simian immunodeficiency virus

General Stability

General Stability Organism
purified enzyme is unstable due to autopropteolysis Simian immunodeficiency virus

Inhibitors

Inhibitors Comment Organism Structure
1,2-epoxy-3-(4-nitrophenoxy)propane i.e. EPNP; irreversible inactivation, half-maximal inactivation at 8.6 mM Simian immunodeficiency virus
amastatin
-
 Simian immunodeficiency virus
chymostatin
-
 Simian immunodeficiency virus
pepstatin A
-
 Simian immunodeficiency virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly-NH2 pH 6, 37°C Simian immunodeficiency virus
3.1
-
 acetyl-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val pH 6, 37°C Simian immunodeficiency virus
4.3
-
 Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2 pH 6, 37°C Simian immunodeficiency virus
4.6
-
 Ac-Ala-Thr-Leu-Asn-Phe-Pro-Ile-Ser-Pro-Ile-Glu-NH2 pH 6, 37°C Simian immunodeficiency virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10812
-
 2 * 10812, electrospray mass spectrometry Simian immunodeficiency virus
11600
-
 2 * 11600, SDS-PAGE Simian immunodeficiency virus
18000 19000 gel filtration Simian immunodeficiency virus

Organism

Organism UniProt Comment Textmining
Simian immunodeficiency virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Simian immunodeficiency virus

Storage Stability

Storage Stability Organism
-70°C, 50 mM sodium acetate, pH 5.0, 5 mM EDTA, 10 mM DTT, 0.35 NaCl, 40% v/v glycerol Simian immunodeficiency virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-Ala-Thr-Leu-Asn-Phe-Pro-Ile-Ser-Pro-Ile-Glu-NH2 + H2O
-
 Simian immunodeficiency virus Ac-Ala-Thr-Leu-Asn-Phe + Pro-Ile-Ser-Pro-Ile-Glu-NH2
-
 ?
Ac-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2 + H2O
-
 Simian immunodeficiency virus Ac-Arg-Ala-Ser-Gln-Asn-Tyr-OH + Pro-Val-Val-NH2
-
 ?
Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly-NH2 + H2O
-
 Simian immunodeficiency virus Ac-Arg-Lys-Ile-Leu + Phe-Leu-Asp-Gly-NH2
-
 ?
Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2 + H2O
-
 Simian immunodeficiency virus Ac-Ser-Gln-Asn-Tyr + Pro-Val-Val-NH2
-
 ?
acetyl-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val + H2O
-
 Simian immunodeficiency virus acetyl-Arg-Ala-Ser-Gln-Asn-Tyr + Pro-Val-Val
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 10812, electrospray mass spectrometry Simian immunodeficiency virus
dimer 2 * 11600, SDS-PAGE Simian immunodeficiency virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 Ac-Ala-Thr-Leu-Asn-Phe-Pro-Ile-Ser-Pro-Ile-Glu-NH2 pH 6, 37°C Simian immunodeficiency virus
1.3
-
 Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly-NH2 pH 6, 37°C Simian immunodeficiency virus
6.2
-
 acetyl-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val pH 6, 37°C Simian immunodeficiency virus
6.4
-
 Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2 pH 6, 37°C Simian immunodeficiency virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2 or Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly as substrate Simian immunodeficiency virus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
 pepstatin A pH 6, 37°C Simian immunodeficiency virus
0.3
-
 chymostatin pH 6, 37°C Simian immunodeficiency virus
0.3
-
 amastatin pH 6, 37°C Simian immunodeficiency virus

pI Value

Organism Comment pI Value Maximum pI Value
Simian immunodeficiency virus isoelectric focusing
-
 8.2