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Literature summary for 3.4.23.B19 extracted from

  • Loymunkong, C.; Sittikul, P.; Songtawee, N.; Wongpanya, R.; Boonyalai, N.
    Yield improvement and enzymatic dissection of Plasmodium falciparum plasmepsin V (2019), Mol. Biochem. Parasitol., 231, 111188 .
    View publication on PubMed
Search for more literature for 3.4.23.B19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
C178A the mutant shows reduced activity compared to the wild type enzyme Plasmodium falciparum
C178S the mutant shows increased activity compared to the wild type enzyme Plasmodium falciparum
D118N the mutant shows reduced activity compared to the wild type enzyme Plasmodium falciparum
D118N/D365N the mutant shows reduced activity compared to the wild type enzyme Plasmodium falciparum
D365N the mutant shows reduced activity compared to the wild type enzyme Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 75% residual activity at 0.5 mM Plasmodium falciparum
Hg2+ about 20-30% residual activity at 0.5 mM Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00342
-
 EMP2 mutant enzyme C178S, at pH 6.5 and 25°C Plasmodium falciparum
0.00374
-
 HRPII mutant enzyme C178S, at pH 6.5 and 25°C Plasmodium falciparum
0.00384
-
 EMP2 wild type enzyme, at pH 6.5 and 25°C Plasmodium falciparum
0.00422
-
 HRPII wild type enzyme, at pH 6.5 and 25°C Plasmodium falciparum
0.00478
-
 EMP2 mutant enzyme C178A, at pH 6.5 and 25°C Plasmodium falciparum
0.00509
-
 HRPII mutant enzyme C178A, at pH 6.5 and 25°C Plasmodium falciparum
0.00957
-
 HRPII mutant enzyme D118N, at pH 6.5 and 25°C Plasmodium falciparum
0.00969
-
 EMP2 mutant enzyme D118N, at pH 6.5 and 25°C Plasmodium falciparum
0.0135
-
 EMP2 mutant enzyme D365N, at pH 6.5 and 25°C Plasmodium falciparum
0.0142
-
 HRPII mutant enzyme D365N, at pH 6.5 and 25°C Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Plasmodium falciparum 5783
-

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8I6Z5 isolate 3D7
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Hitrap Q column chromatography Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0009
-
 mutant enzyme D118N/D365N, with HRPII as substrate, at pH 6.5 and 25°C Plasmodium falciparum
0.01
-
 mutant enzyme D118N/D365N, with EMP2 as substrate, at pH 6.5 and 25°C Plasmodium falciparum
0.022
-
 mutant enzyme D365N, with HRPII as substrate, at pH 6.5 and 25°C Plasmodium falciparum
0.027
-
 mutant enzyme D365N, with EMP2 as substrate, at pH 6.5 and 25°C Plasmodium falciparum
0.041
-
 mutant enzyme D118N, with HRPII as substrate, at pH 6.5 and 25°C Plasmodium falciparum
0.05
-
 mutant enzyme D118N, with EMP2 as substrate, at pH 6.5 and 25°C Plasmodium falciparum
0.055
-
 mutant enzyme C178A, with HRPII as substrate, at pH 6.5 and 25°C Plasmodium falciparum
0.083
-
 wild type enzyme, with HRPII as substrate, at pH 6.5 and 25°C Plasmodium falciparum
0.088
-
 mutant enzyme C178A, with EMP2 as substrate, at pH 6.5 and 25°C Plasmodium falciparum
0.09
-
 mutant enzyme C178S, with HRPII as substrate, at pH 6.5 and 25°C Plasmodium falciparum
0.09
-
 wild type enzyme, with EMP2 as substrate, at pH 6.5 and 25°C Plasmodium falciparum
0.114
-
 mutant enzyme C178S, with EMP2 as substrate, at pH 6.5 and 25°C Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
EMP2 + H2O i.e. Dabcyl-RYVRILSETEE-Edans Plasmodium falciparum ?
-
 ?
HRPII + H2O i.e. Dabcyl-LNKRLLHETQE-Edans Plasmodium falciparum ?
-
 ?
additional information no activity with DABCYL-KAVHYLEHNVKE-EDANS and Dabcyl-RYVAIASATEE-Edans Plasmodium falciparum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Plasmodium falciparum

Synonyms

Synonyms Comment Organism
PMV
-
 Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.19
-
 HRPII mutant enzyme D365N, at pH 6.5 and 25°C Plasmodium falciparum
2.26
-
 EMP2 mutant enzyme D365N, at pH 6.5 and 25°C Plasmodium falciparum
2.35
-
 HRPII mutant enzyme D118N, at pH 6.5 and 25°C Plasmodium falciparum
2.56
-
 EMP2 mutant enzyme D118N, at pH 6.5 and 25°C Plasmodium falciparum
2.79
-
 HRPII mutant enzyme C178A, at pH 6.5 and 25°C Plasmodium falciparum
2.97
-
 HRPII wild type enzyme, at pH 6.5 and 25°C Plasmodium falciparum
3.01
-
 EMP2 mutant enzyme C178A, at pH 6.5 and 25°C Plasmodium falciparum
3.12
-
 EMP2 wild type enzyme, at pH 6.5 and 25°C Plasmodium falciparum
3.35
-
 EMP2 mutant enzyme C178S, at pH 6.5 and 25°C Plasmodium falciparum
3.36
-
 HRPII mutant enzyme C178S, at pH 6.5 and 25°C Plasmodium falciparum

General Information

General Information Comment Organism
physiological function the enzyme processes proteins for export into the host erythrocyte and plays a crucial role in parasite virulence and survival Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
154
-
 HRPII mutant enzyme D365N, at pH 6.5 and 25°C Plasmodium falciparum
167
-
 EMP2 mutant enzyme D365N, at pH 6.5 and 25°C Plasmodium falciparum
245
-
 HRPII mutant enzyme D118N, at pH 6.5 and 25°C Plasmodium falciparum
264
-
 EMP2 mutant enzyme D118N, at pH 6.5 and 25°C Plasmodium falciparum
549
-
 HRPII mutant enzyme C178A, at pH 6.5 and 25°C Plasmodium falciparum
630
-
 EMP2 mutant enzyme C178A, at pH 6.5 and 25°C Plasmodium falciparum
704
-
 HRPII wild type enzyme, at pH 6.5 and 25°C Plasmodium falciparum
813
-
 EMP2 wild type enzyme, at pH 6.5 and 25°C Plasmodium falciparum
898
-
 HRPII mutant enzyme C178S, at pH 6.5 and 25°C Plasmodium falciparum
981
-
 EMP2 mutant enzyme C178S, at pH 6.5 and 25°C Plasmodium falciparum