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Literature summary for 3.4.23.B19 extracted from
- Biochemical characterization of plasmepsin V from Plasmodium vivax Thailand isolates Substrate specificity and enzyme inhibition (2015), Mol. Biochem. Parasitol., 204, 51-63 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as thioredoxin fusion proteins in Escherichia coli BL21 (DE3) cells | Plasmodium vivax |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| menisporopsin A | 10-30% inhibition at 0.01 mM, 40-60% inhibition at 0.1 mM | Plasmodium vivax |  |
| additional information | not inhibited by pepstatin A and nelfinavir | Plasmodium vivax | |
| phenylmethylsulfonyl fluoride | about 20% inhibition at 0.01 mM, 50-70% inhibition at 0.1 mM | Plasmodium vivax | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00066 | - |
EMP2 | proenzyme, at pH 6.5 and 25°C | Plasmodium vivax | |
| 0.00093 | - |
HRPII | proenzyme, at pH 6.5 and 25°C | Plasmodium vivax | |
| 0.00096 | - |
HRPII | mature enzyme, at pH 6.5 and 25°C | Plasmodium vivax | |
| 0.00139 | - |
EMP2 | mature enzyme, at pH 6.5 and 25°C | Plasmodium vivax |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium vivax | A0A0H4FQ60 | - |
- |
| Plasmodium vivax Thai | A0A0H4FQ60 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography | Plasmodium vivax |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| using a 10-volume rapid dilution into 20 mM Tris-HCl pH 8.0, 1 mM reduced glutathione, 0.1 mM oxidized glutathione, 0.15 M NaCl, and 10% (v/v) glycerol with stirring at 25°C for 24 h | Plasmodium vivax |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Dabcyl-LNKRELHETQE-Edans + H2O | about 50% activity compared to the HRPII peptide Dabcyl-LNKRLLHETQE-Edans | Plasmodium vivax | ? | - |
? | |
| Dabcyl-LNKRELHETQE-Edans + H2O | about 50% activity compared to the HRPII peptide Dabcyl-LNKRLLHETQE-Edans | Plasmodium vivax Thai | ? | - |
? | |
| Dabcyl-LNKRKLHETQE-Edans + H2O | about 130% activity compared to the HRPII peptide Dabcyl-LNKRLLHETQE-Edans | Plasmodium vivax | ? | - |
? | |
| Dabcyl-LNKRKLHETQE-Edans + H2O | about 130% activity compared to the HRPII peptide Dabcyl-LNKRLLHETQE-Edans | Plasmodium vivax Thai | ? | - |
? | |
| Dabcyl-LNKRLLEETQE-Edans + H2O | about 50% activity compared to the HRPII peptide Dabcyl-LNKRLLHETQE-Edans | Plasmodium vivax | ? | - |
? | |
| Dabcyl-LNKRLLEETQE-Edans + H2O | about 50% activity compared to the HRPII peptide Dabcyl-LNKRLLHETQE-Edans | Plasmodium vivax Thai | ? | - |
? | |
| Dabcyl-LNKRLLSETQE-Edans + H2O | about 180% activity compared to the HRPII peptide Dabcyl-LNKRLLHETQE-Edans | Plasmodium vivax | ? | - |
? | |
| Dabcyl-LNKRLLSETQE-Edans + H2O | about 180% activity compared to the HRPII peptide Dabcyl-LNKRLLHETQE-Edans | Plasmodium vivax Thai | ? | - |
? | |
| Dabcyl-LNKRLLVETQE-Edans + H2O | about 150% activity compared to the HRPII peptide Dabcyl-LNKRLLHETQE-Edans | Plasmodium vivax | ? | - |
? | |
| Dabcyl-RYVRELSETEE-Edans + H2O | about 10-25% activity compared to the EMP2 peptide Dabcyl-RYVRILSETEE-Edans | Plasmodium vivax | ? | - |
? | |
| Dabcyl-RYVRILEETEE-Edans + H2O | about 7-30% activity compared to the EMP2 peptide Dabcyl-RYVRILSETEE-Edans | Plasmodium vivax | ? | - |
? | |
| Dabcyl-RYVRILVETEE-Edans + H2O | about 8-30% activity compared to the EMP2 peptide Dabcyl-RYVRILSETEE-Edans | Plasmodium vivax | ? | - |
? | |
| Dabcyl-RYVRKLSETEE-Edans + H2O | about 70% activity compared to the EMP2 peptide Dabcyl-RYVRILSETEE-Edans | Plasmodium vivax | ? | - |
? | |
| EMP2 + H2O | i.e. Dabcyl-RYVRILSETE-Edans, most efficient substrate. Replacing Ser by Val or Glu of the PfEMP2 peptide P1' position markedly reduces the enzyme activity of the enzyme | Plasmodium vivax | ? | - |
? | |
| HRPII + H2O | i.e. Dabcyl-LNKRLLHETQE-Edans. Replacing His by Val or Ser of the HRPII peptide P1' position increases the cleavage activity | Plasmodium vivax | ? | - |
? | |
| additional information | no activity with DABCYL-KAVHYLEHNVKE-EDANS and Dabcyl-RYVAIASATEE-Edans | Plasmodium vivax | ? | - |
? | |
| additional information | no activity with DABCYL-KAVHYLEHNVKE-EDANS and Dabcyl-RYVAIASATEE-Edans | Plasmodium vivax Thai | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61700, calculated from amino acid sequence | Plasmodium vivax |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PMV | - |
Plasmodium vivax |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
HRPII | mature enzyme, at pH 6.5 and 25°C | Plasmodium vivax | |
| 0.06 | - |
HRPII | proenzyme, at pH 6.5 and 25°C | Plasmodium vivax | |
| 0.121 | - |
EMP2 | proenzyme, at pH 6.5 and 25°C | Plasmodium vivax | |
| 0.203 | - |
EMP2 | mature enzyme, at pH 6.5 and 25°C | Plasmodium vivax |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Plasmodium vivax |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 55 | - |
HRPII | mature enzyme, at pH 6.5 and 25°C | Plasmodium vivax | |
| 66 | - |
HRPII | proenzyme, at pH 6.5 and 25°C | Plasmodium vivax | |
| 146 | - |
EMP2 | mature enzyme, at pH 6.5 and 25°C | Plasmodium vivax | |
| 183 | - |
EMP2 | proenzyme, at pH 6.5 and 25°C | Plasmodium vivax |
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