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Literature summary for 3.4.23.B19 extracted from

  • Boonyalai, N.; Sittikul, P.; Yuvaniyama, J.
    Plasmodium falciparum plasmepsin V (PfPMV) Insights into recombinant expression, substrate specificity and active site structure (2015), Mol. Biochem. Parasitol., 201, 5-15 .
    View publication on PubMed
Search for more literature for 3.4.23.B19

Cloned(Commentary)

Cloned (Comment) Organism
expressed as thioredoxin fusion proteins in Escherichia coli L21(DE3) cells Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
additional information the truncated enzyme PMVm84 is not inhibited by pepstatin A Plasmodium falciparum
nelfinavir the truncated enzyme PMVm84 exhibits about 53% residual activity at 0.1 mM Plasmodium falciparum
phenylmethylsulfonyl fluoride the truncated enzyme PMVm84 exhibits about 70% residual activity at 0.1 mM Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00021
-
 EMP2 truncated enzyme PMVp37, at pH 6.5 and 37°C Plasmodium falciparum
0.00029
-
 EMP2 truncated enzyme PMVm84, at pH 6.5 and 37°C Plasmodium falciparum
0.00038
-
 HRPII truncated enzyme PMVm84, at pH 6.5 and 37°C Plasmodium falciparum
0.00114
-
 HRPII truncated enzyme PMVp37, at pH 6.5 and 37°C Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8I6Z5 isolate 3D7
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Hitrap Q column chromatography Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Dabcyl-RYVRELSETE-Edans + H2O about 68% activity compared to the EMP2 peptide Dabcyl-RYVRILSETE-Edans Plasmodium falciparum ?
-
 ?
Dabcyl-RYVRILEETE-Edans + H2O about 28% activity compared to the EMP2 peptide Dabcyl-RYVRILSETE-Edans Plasmodium falciparum ?
-
 ?
Dabcyl-RYVRILVETE-Edans + H2O about 28% activity compared to the EMP2 peptide Dabcyl-RYVRILSETE-Edans Plasmodium falciparum ?
-
 ?
Dabcyl-RYVRKLSETE-Edans + H2O about 70% activity compared to the EMP2 peptide Dabcyl-RYVRILSETE-Edans Plasmodium falciparum ?
-
 ?
EMP2 + H2O i.e. Dabcyl-RYVRILSETE-Edans Plasmodium falciparum Dabcyl-RYVRIL + SETE-Edans
-
 ?
HRPII + H2O i.e. Dabcyl-LNKRLLHETQE-Edans Plasmodium falciparum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 54600, truncated enzyme PMVm84, SDS-PAGE Plasmodium falciparum
? x * 59700, truncated enzyme PMVp37, SDS-PAGE Plasmodium falciparum

Synonyms

Synonyms Comment Organism
PMV
-
 Plasmodium falciparum
PMVm84 truncated enzyme Plasmodium falciparum
PMVp37 truncated enzyme Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 HRPII truncated enzyme PMVm84, at pH 6.5 and 37°C Plasmodium falciparum
0.13
-
 HRPII truncated enzyme PMVp37, at pH 6.5 and 37°C Plasmodium falciparum
0.19
-
 EMP2 truncated enzyme PMVm84, at pH 6.5 and 37°C Plasmodium falciparum
0.23
-
 EMP2 truncated enzyme PMVp37, at pH 6.5 and 37°C Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 more than 50% activity between pH 5.5 and 7.0 Plasmodium falciparum

General Information

General Information Comment Organism
metabolism the enzyme is responsible for the cleavage of the Plasmodium export element (PEXEL) motif at the N-terminus of several hundreds of the exported proteins Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
110
-
 HRPII truncated enzyme PMVp37, at pH 6.5 and 37°C Plasmodium falciparum
310
-
 HRPII truncated enzyme PMVm84, at pH 6.5 and 37°C Plasmodium falciparum
6500
-
 EMP2 truncated enzyme PMVm84, at pH 6.5 and 37°C Plasmodium falciparum
10900
-
 EMP2 truncated enzyme PMVp37, at pH 6.5 and 37°C Plasmodium falciparum