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Literature summary for 3.4.23.B14 extracted from

  • Liu, P.; Robbins, A.H.; Marzahn, M.R.; McClung, S.H.; Yowell, C.A.; Stevens, S.M.; Dame, J.B.; Dunn, B.M.
    Enzymatic characterization of recombinant food vacuole plasmepsin 4 from the rodent malaria parasite Plasmodium berghei (2015), PLoS ONE, 10, e0141758 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.B14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) pLysS cells Plasmodium berghei

Inhibitors

Inhibitors Comment Organism Structure
KPFEL-CH2-NH-AWT
-
 Plasmodium berghei
KPFSL-CH2-NH-LQF
-
 Plasmodium berghei
KPLEF-CH2-NH-FRV
-
 Plasmodium berghei
KPLEF-CH2-NH-YRV
-
 Plasmodium berghei
KPVEF-CH2-NH-RQT
-
 Plasmodium berghei
KPYEF-CH2-NH-RQF
-
 Plasmodium berghei
Lys-Pro-norleucine-Ser-norleucine-CH2-NH-Leu-Gln-Ile
-
 Plasmodium berghei
pepstatin A competitive inhibitor Plasmodium berghei
Ro40-4388 competitive inhibitor Plasmodium berghei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 Lys-Pro-Ile-Leu-Phe-4-nitrophenylalanine-Arg-Leu at pH 5.5 and 37°C Plasmodium berghei
0.0038
-
 Lys-Pro-Ile-Gln-Phe-4-nitrophenylalanine-Arg-Leu at pH 5.5 and 37°C Plasmodium berghei

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole
-
 Plasmodium berghei 5773
-

Organism

Organism UniProt Comment Textmining
Plasmodium berghei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography and Superdex 75 gel filtration Plasmodium berghei

Renatured (Commentary)

Renatured (Comment) Organism
-
 Plasmodium berghei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Lys-Pro-Ile-Gln-Phe-4-nitrophenylalanine-Arg-Leu + H2O
-
 Plasmodium berghei Lys-Pro-Ile-Gln-Phe + 4-nitrophenylalanine-Arg-Leu
-
 ?
Lys-Pro-Ile-Leu-Phe-4-nitrophenylalanine-Arg-Leu + H2O
-
 Plasmodium berghei Lys-Pro-Ile-Leu-Phe + 4-nitrophenylalanine-Arg-Leu
-
 ?
additional information the enzyme cleaves preferably behind phenylalanine. The hydrolysis rates of the leucine and norleucine pools are 34% and 42% of that of the phenylalanine pool, respectively. In addition, no levels of hydrolysis are also detected for peptide pools containing four other P1 amino acid substitutes, asparagine (25%), glutamine (20%), tyrosine (18%) and tryptophan (17%). Hydrolyzed peptide products from the other 12 peptide pools, however, were barely detected Plasmodium berghei ?
-
 ?
additional information the plasmepsin 4 zymogen exhibits catalytic activity regardless of the presence of the pro-segment. Auto-maturation of the zymogen to mature enzyme is carried out at pH 4.5, 5.0, and 5.5 Plasmodium berghei ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, mature enzyme, SDS-PAGE Plasmodium berghei
monomer 1 * 43000, zymogen, SDS-PAGE Plasmodium berghei

Synonyms

Synonyms Comment Organism
plasmepsin 4
-
 Plasmodium berghei
PM4
-
 Plasmodium berghei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Plasmodium berghei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24.5
-
 Lys-Pro-Ile-Gln-Phe-4-nitrophenylalanine-Arg-Leu at pH 5.5 and 37°C Plasmodium berghei
34.9
-
 Lys-Pro-Ile-Leu-Phe-4-nitrophenylalanine-Arg-Leu at pH 5.5 and 37°C Plasmodium berghei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 the mature enzyme shows highest catalytic activity at pH 5.0 and pH 5.5 with a 5 min pre-incubation Plasmodium berghei
5.5
-
 the zymogen shows highest catalytic activity at pH 5.0 and pH 5.5 with a 5 min pre-incubation Plasmodium berghei

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 5.5 the enzyme shows 75-90% of the maximal catalytic activity within 30 min pre-incubation at pH 5.0-5.5 Plasmodium berghei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000011
-
 pepstatin A at pH 5.0 and 37°C Plasmodium berghei
0.00000012
-
 KPLEF-CH2-NH-YRV at pH 5.0 and 37°C Plasmodium berghei
0.0000014
-
 KPLEF-CH2-NH-FRV at pH 5.0 and 37°C Plasmodium berghei
0.000135
-
 Ro40-4388 at pH 5.0 and 37°C Plasmodium berghei
0.000375
-
 Lys-Pro-norleucine-Ser-norleucine-CH2-NH-Leu-Gln-Ile at pH 5.0 and 37°C Plasmodium berghei
0.000379
-
 KPFSL-CH2-NH-LQF at pH 5.0 and 37°C Plasmodium berghei
0.000502
-
 KPVEF-CH2-NH-RQT at pH 5.0 and 37°C Plasmodium berghei
0.0039
-
 KPYEF-CH2-NH-RQF at pH 5.0 and 37°C Plasmodium berghei
0.0081
-
 KPFEL-CH2-NH-AWT at pH 5.0 and 37°C Plasmodium berghei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6400
-
 Lys-Pro-Ile-Gln-Phe-4-nitrophenylalanine-Arg-Leu at pH 5.5 and 37°C Plasmodium berghei
16500
-
 Lys-Pro-Ile-Leu-Phe-4-nitrophenylalanine-Arg-Leu at pH 5.5 and 37°C Plasmodium berghei