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Literature summary for 3.4.23.B14 extracted from
- Plasmodium vivax: collaborative roles for plasmepsin 4 and vivapains in hemoglobin hydrolysis (2011), Exp. Parasitol., 128, 127-132.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Plasmodium vivax |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| pepstatin | efficinet inhibition of autoprocessing to mature PM4 | Plasmodium vivax |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 44000, SDs-PAGE of preprotein, x * 37000, SDS-PAGE of mature protein | Plasmodium vivax |
| 44000 | - |
x * 44000, SDs-PAGE of preprotein, x * 37000, SDS-PAGE of mature protein | Plasmodium vivax |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium vivax | O60989 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | processing of PM4 into a mature enzyme occurrs through autocatalytic activity under acidic conditions in a pepstatin A-sensitive manner, in which process a portion of prodomain is essential for correct folding. Cysteine proteases vivapain-2 and vivapain-3 mediate the processing of PvPM4 into a mature enzyme | Plasmodium vivax |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| bacterially expressed recombinant PvPM4 is insoluble, but it is easily refolded into a soluble protein | Plasmodium vivax |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| denatured human hemoglobin + H2O | preferred over native human hemoglobin. PvPM4 acts synergistically with cysteine proteases vivapain-2 and vivapain-3 in the hydrolysis of hemoglobin | Plasmodium vivax | ? | - |
? | |
| native human gemoglobin + H2O | poor substrate | Plasmodium vivax | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000, SDs-PAGE of preprotein, x * 37000, SDS-PAGE of mature protein | Plasmodium vivax |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| plasmepsin 4 | - |
Plasmodium vivax |
| PM4 | - |
Plasmodium vivax |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
highly stable under acidic and neutral conditions | Plasmodium vivax |
| 50 | - |
unstable | Plasmodium vivax |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 5.5 | broad | Plasmodium vivax |
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Release 2023.1 (January 2023)
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