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Literature summary for 3.4.23.B14 extracted from
- Investigating alternative acidic proteases for H/D exchange coupled to mass spectrometry: plasmepsin 2 but not plasmepsin 4 is active under quenching conditions (2010), J. Am. Soc. Mass Spectrom., 21, 76-79.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | PSM4 is unusable for structural studies by hydrogen/deuterium exchange coupled to mass spectrometry, DXMS, since the method requires the use of proteases working at acidic pH and low temperatures, in contrast to PSM2, EC 3.4.23.39 | Plasmodium falciparum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | maturation of PSM4 is efficient after 5 min at pH 4.5 and 37°C, PSM4 is partly cleaved after Phe109, Phe110, or Phe81 leading to proteins of 38218 Da, 38071 Da, and 41508 Da, respectively, cleavage is not terminated after 7 h | Plasmodium falciparum |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | PSM4 is unusable for structural studies by hydrogen/deuterium exchange coupled to mass spectrometry, DXMS, since the method requires the use of proteases working at acidic pH and low temperatures | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| plasmepsin 4 | - |
Plasmodium falciparum |
| PSM4 | - |
Plasmodium falciparum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Plasmodium falciparum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
PSM4 is inactive at 0 °C | Plasmodium falciparum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
assay at | Plasmodium falciparum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
PSM4 is inactive at pH 2.2 | Plasmodium falciparum |
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