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Literature summary for 3.4.23.B11 extracted from

  • Hartl, M.; Mayr, F.; Rethwilm, A.; Woehrl, B.
    Biophysical and enzymatic properties of the simian and prototype foamy virus reverse transcriptases (2010), Retrovirology, 7, 05.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.B11

Activating Compound

Activating Compound Comment Organism Structure
NaCl assay at 3 M, no activity at salt concentrations of 0.2-0.4 M Na. In vitro dimerization of the protease domain is inducible at high salt concentrations. This effect might be caused by a hydrophobic dimerization interface, which under high ionic strength disfavors the monomeric state Simian foamy virus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Simian foamy virus
expression in Escherichia coli prototype foamy virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84500
-
 gel filtration prototype foamy virus
86500
-
 1 * 86500, calculated prototype foamy virus

Organism

Organism UniProt Comment Textmining
prototype foamy virus
-
-
-
Simian foamy virus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GB1-GFP + H2O peptide substrate, harboring the simian foamy virus mac reverse transcriptase-viral integrase cleavage site of the Pol polyprotein, ATQGSYVVHCNTTP, between immunoglobulin binding domain B1 of streptococcal protein G, GB1, and green fluorescent protein, GFP Simian foamy virus ? cleavage occurs at YVVH-CNTT ?
GB1-GFP + H2O peptide substrate, harboring the simian foamy virus mac reverse transcriptase-viral integrase cleavage site of the Pol polyprotein, ATQGSYVVHCNTTP, between immunoglobulin binding domain B1 of streptococcal protein G, GB1, and green fluorescent protein, GFP prototype foamy virus ? cleavage occurs at YVVH-CNTT ?

Subunits

Subunits Comment Organism
monomer 1 * 86500, calculated prototype foamy virus
More under native conditions, no detection of dimer prototype foamy virus