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Literature summary for 3.4.23.B11 extracted from

  • Roy, J.; Linial, M.L.
    Role of the foamy virus Pol cleavage site in viral replication (2007), J. Virol., 81, 4956-4962.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.B11

Cloned(Commentary)

Cloned (Comment) Organism
Virion-associated RT assay is performed in BHK cells, ATCC CCL-10. Analysis of titers of wild-type and mutant proviruses transiently transfected into HEK-293T cells. Titers of Pol cleavage site mutants after infection measured in HEL cells. Human spumaretrovirus

Protein Variants

Protein Variants Comment Organism
additional information PCR mutagenesis, cleavage site mutants of Human foamy virus protease PR generated using the full-length viral clone pcHFV13 Human spumaretrovirus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Gag protein precursor + H2O Human spumaretrovirus
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 ?
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 ?

Organism

Organism UniProt Comment Textmining
Human spumaretrovirus P14349 Human foamy virus HFV
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Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant forms, SDS-PAGE Human spumaretrovirus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 sequence at the cleavage sites in wild-type Human foamy virus and in cleavage site mutants indicated, generation of Pol cleavage site mutants, expression and cleavage activity of Human foamy virus protease PR analyzed by Western Blot, reduced levels of PR activity observed, association between reduced enzyme activity and defects in replication of cleavage site mutant viruses shown Human spumaretrovirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gag protein precursor + H2O
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 Human spumaretrovirus ?
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 ?
Pol protein precursor + H2O analysis of cleavage sites of Human foamy virus protease PR, mutations around the cleavage site of the Human foamy virus Pol precursor protein shown to affect enzymatic activities of the protease PR Human spumaretrovirus ?
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 ?

Synonyms

Synonyms Comment Organism
human foamy virus protease PR
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 Human spumaretrovirus