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Literature summary for 3.4.23.B10 extracted from

  • Scheifele, L.Z.; Ryan, E.P.; Parent, L.J.
    Detailed mapping of the nuclear export signal in the Rous sarcoma virus Gag protein (2005), J. Virol., 79, 8732-8741.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.B10

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant Gag polyprotein fused to GFP in QT6 cells Rous sarcoma virus

Protein Variants

Protein Variants Comment Organism
L180A/L184A/V187A site-directed mutagenesis of the Gag polyprotein p10 sequence residues, the mutant protein shows normal nucleocytoplasmic trafficking and localization at the cell plasmamembrane and in the cytosol Rous sarcoma virus
L219A site-directed mutagenesis of the Gag polyprotein p10 sequence residue leads to accumulation of the mutant protein in the nucleus and reduced virus assembly, overview Rous sarcoma virus
L229A site-directed mutagenesis of the Gag polyprotein p10 sequence residue leads to accumulation of the mutant protein in the nucleus and reduced virus assembly, overview Rous sarcoma virus
additional information delocalization mutations of the NES motif of p10 sequence shows that the motif can be located anywhere within the Gag polyprotein without loosing its function in translocation, overview Rous sarcoma virus
V225A site-directed mutagenesis of the Gag polyprotein p10 sequence residue leads to accumulation of the mutant protein in the nucleus and reduced virus assembly, overview Rous sarcoma virus
W222A site-directed mutagenesis of the Gag polyprotein p10 sequence residue leads to accumulation of the mutant protein in the nucleus and reduced virus assembly, overview Rous sarcoma virus

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme sequence, or p10 sequence, contains a leptomycin B-dependent nuclear-export-signal, NES Rous sarcoma virus
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Gag polyprotein precursor + H2O Rous sarcoma virus cleavage into several different mature proteins, processing by the p10 sequence portion of the Gag-polyprotein C-terminus ?
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 ?
additional information Rous sarcoma virus the enzyme is an intrinsic part of the virus assembly responsible for coordination of the process ?
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 ?

Organism

Organism UniProt Comment Textmining
Rous sarcoma virus
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 RSV
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gag polyprotein precursor + H2O cleavage into several different mature proteins, processing by the p10 sequence portion of the Gag-polyprotein C-terminus Rous sarcoma virus ?
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 ?
additional information the enzyme is an intrinsic part of the virus assembly responsible for coordination of the process Rous sarcoma virus ?
-
 ?
additional information the soluble export receptor Crm1, and the nuclear pore proteins Nup214 and Nup98 are important cofactors of the enzyme involved in the export of the RSV GAg protein from the nucleus, overview Rous sarcoma virus ?
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 ?

Subunits

Subunits Comment Organism
More the enzyme sequence, or p10 sequence of the Gag polyprotein, contains a leptomycin B-dependent nuclear-export-signal, NES, involving residues L219, W222, V225, and L229, the NES sequence is positionally independent and transferable, overview Rous sarcoma virus

Synonyms

Synonyms Comment Organism
Gag polyprotein p10 sequence
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 Rous sarcoma virus