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Literature summary for 3.4.23.B10 extracted from
- Rous sarcoma virus retropepsin and avian myeloblastosis virus retropepsin (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 163-166.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Avian myeloblastosis virus |
| expression of wild-type and mutant enzymes in Escherichia coli in inclusion bodies, expression of N-terminally His-tagged enzyme in Escherichia coli in the soluble fraction | Rous sarcoma virus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| free or ligated enzyme, structure analysis in comparison to HIV-1 retropepsin | Avian myeloblastosis virus |
| free or ligated wild-type and mutant enzymes, structure analysis in comparison to HIV-1 retropepsin | Rous sarcoma virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S38T/I42D/I44V/M73V/A100L/V104T/R105P/G106V/S107N | site-directed mutagenesis leading to the mutant RSV S9 protease, which is active with HIV-1 retropepsin substrates and inhibitable by HIV-1 retropepsin-specific inhibitors in contrast to the wild-type enzyme | Avian myeloblastosis virus |
| S38T/I42D/I44V/M73V/A100L/V104T/R105P/G106V/S107N | site-directed mutagenesis leading to the mutant RSV S9 protease, which is active with HIV-1 retropepsin substrates and inhibitable by HIV-1 retropepsin-specific inhibitors in contrast to the wild-type enzyme | Rous sarcoma virus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme contains no metal ions or disulfide bridges | Avian myeloblastosis virus | |
| additional information | the enzyme contains no metal ions or disulfide bridges | Rous sarcoma virus | |
| NaCl | activates optimally at 2.4 M | Avian myeloblastosis virus |  |
| NaCl | activates optimally at 2.4 M | Rous sarcoma virus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
- |
Avian myeloblastosis virus |
| 27000 | - |
- |
Rous sarcoma virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gag precursor polyprotein + H2O | Avian myeloblastosis virus | cleavage into several different mature proteins, processing by the avian myoblastosis retropepsin, which is part of the Gag-polyprotein and located at the C-terminus | ? | - |
? | |
| gag precursor polyprotein + H2O | Rous sarcoma virus | cleavage into several different mature proteins, processing by the p15 protein or rous sarcoma virus retropepsin, which is part of the Gag-polyprotein and located at the C-terminus | ? | - |
? | |
| gag precursor polyprotein + H2O | Avian myeloblastosis virus AMV | cleavage into several different mature proteins, processing by the avian myoblastosis retropepsin, which is part of the Gag-polyprotein and located at the C-terminus | ? | - |
? | |
| Gag-Pol precursor polyprotein + H2O | Avian myeloblastosis virus | cleavage into several different mature proteins | ? | - |
? | |
| Gag-Pol precursor polyprotein + H2O | Rous sarcoma virus | cleavage into several different mature proteins | ? | - |
? | |
| Gag-Pol precursor polyprotein + H2O | Avian myeloblastosis virus AMV | cleavage into several different mature proteins | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Avian myeloblastosis virus | - |
AMV | - |
| Avian myeloblastosis virus AMV | - |
AMV | - |
| Rous sarcoma virus | - |
RSV | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the enzyme is part of the Gag-polyprotein and located at the C-terminus, and autocatalytically cleaves itself to the active homodimer | Avian myeloblastosis virus |
| proteolytic modification | the enzyme is part of the Gag-polyprotein and located at the C-terminus, and autocatalytically cleaves itself to the active homodimer | Rous sarcoma virus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from whole virus | Avian myeloblastosis virus |
| recombinant wild-type and mutant enzymes from Escherichia coli with refolding from inclusion bodies to over 95% purity, N-terminally His-tagged enzyme from Escherichia coli soluble fraction by nickel affinity chromatography | Rous sarcoma virus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| The cleavage sequence in the natural substrate NC-PR is PPAVS-/-LAMTMRR. The activity can be improved by substitution by Trp, Tyr, Phe, Leu, Arg, Glu, His or Ala in P1, Tyr in P3', and Arg, Phe, Asn or His in P3 | substrate binding structure and mechanism | Avian myeloblastosis virus | |
| The cleavage sequence in the natural substrate NC-PR is PPAVS-/-LAMTMRR. The activity can be improved by substitution by Trp, Tyr, Phe, Leu, Arg, Glu, His or Ala in P1, Tyr in P3', and Arg, Phe, Asn or His in P3 | substrate binding structure and mechanism | Rous sarcoma virus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| wild-type and mutant enzymes expressed in Escherichia coli, refolding from inclusion bodies | Rous sarcoma virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gag precursor polyprotein + H2O | cleavage into several different mature proteins | Avian myeloblastosis virus | ? | - |
? | |
| gag precursor polyprotein + H2O | cleavage into several different mature proteins | Rous sarcoma virus | ? | - |
? | |
| gag precursor polyprotein + H2O | cleavage into several different mature proteins, processing by the avian myoblastosis retropepsin, which is part of the Gag-polyprotein and located at the C-terminus | Avian myeloblastosis virus | ? | - |
? | |
| gag precursor polyprotein + H2O | cleavage into several different mature proteins, processing by the p15 protein or rous sarcoma virus retropepsin, which is part of the Gag-polyprotein and located at the C-terminus | Rous sarcoma virus | ? | - |
? | |
| gag precursor polyprotein + H2O | cleavage into several different mature proteins | Avian myeloblastosis virus AMV | ? | - |
? | |
| gag precursor polyprotein + H2O | cleavage into several different mature proteins, processing by the avian myoblastosis retropepsin, which is part of the Gag-polyprotein and located at the C-terminus | Avian myeloblastosis virus AMV | ? | - |
? | |
| Gag-Pol precursor polyprotein + H2O | cleavage into several different mature proteins | Avian myeloblastosis virus | ? | - |
? | |
| Gag-Pol precursor polyprotein + H2O | cleavage into several different mature proteins | Rous sarcoma virus | ? | - |
? | |
| Gag-Pol precursor polyprotein + H2O | cleavage into several different mature proteins | Avian myeloblastosis virus AMV | ? | - |
? | |
| additional information | the Rous sarcoma virus retropepsin differs by only two amino acid residues from the avian myeloblastosis virus retropepsin, both are biochemically indistinguishable, overview | Avian myeloblastosis virus | ? | - |
? | |
| additional information | the Rous sarcoma virus retropepsin differs by only two amino acid residues from the avian myeloblastosis virus retropepsin, both are biochemically indistinguishable, overview | Rous sarcoma virus | ? | - |
? | |
| additional information | the Rous sarcoma virus retropepsin differs by only two amino acid residues from the avian myeloblastosis virus retropepsin, both are biochemically indistinguishable, overview | Avian myeloblastosis virus AMV | ? | - |
? | |
| PATVLTVALRR + H2O | synthetic peptide substrate, required features, overview | Avian myeloblastosis virus | PATVL + TVALRR | - |
? | |
| PATVLTVALRR + H2O | synthetic peptide substrate, required features, overview | Rous sarcoma virus | PATVL + TVALRR | - |
? | |
| PPAVSLAMTMRR + H2O | synthetic peptide substrate, required features, overview | Avian myeloblastosis virus | PPAVS + LAMTMRR | - |
? | |
| PPAVSLAMTMRR + H2O | synthetic peptide substrate, required features, overview | Rous sarcoma virus | PPAVS + LAMTMRR | - |
? | |
| PPAVSLAMTMRR + H2O | synthetic peptide substrate, required features, overview | Avian myeloblastosis virus AMV | PPAVS + LAMTMRR | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | homodimer, the active site and substrate binding site is located in the dimer interface cleft | Avian myeloblastosis virus |
| dimer | homodimer, the active site and substrate binding site is located in the dimer interface cleft | Rous sarcoma virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| avian myeloblastosis virus retropepsin | - |
Avian myeloblastosis virus |
| More | the enzyme belongs to the A2 peptidase family | Avian myeloblastosis virus |
| More | the enzyme belongs to the A2 peptidase family | Rous sarcoma virus |
| p15 protein | - |
Rous sarcoma virus |
| Rous sarcoma virus retropepsin | - |
Rous sarcoma virus |
| RSV/AMV retropepsin | - |
Avian myeloblastosis virus |
| RSV/AMV retropepsin | - |
Rous sarcoma virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | - |
- |
Avian myeloblastosis virus |
| 5.9 | - |
- |
Rous sarcoma virus |
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