Literature summary for 3.4.23.B10 extracted from

Cameron, C.E.; Ridky, T.W.; Shulenin, S.; Leis, J.; Weber, I.T.; Copeland, T.; Wlodawer, A.; Burstein, H.; Bizub-Bender, D.; Skalka, A.M.
Mutational analysis of the substrate binding pockets of the Rous sarcoma virus and human immunodeficiency virus-1 proteases (1994), J. Biol. Chem., 269, 11170-11177.

Search for more literature for 3.4.23.B10

Protein Variants

Protein Variants	Comment	Organism
A100L	mutant enzyme shows 1% of the activity with PPAVSLAMTMRR as substrate compared to activity of the wild-type enzyme	Rous sarcoma virus
DELTAN61-Q63	mutant enzyme shows 245% of the activity with PPAVSLAMTMRR as substrate compared to activity of the wild-type enzyme	Rous sarcoma virus
I42D	mutant enzyme shows 62% of the activity with PPAVSLAMTMRR as substrate compared to activity of the wild-type enzyme. Mutant enzyme exhibits HIV-1 protease specificity for P2- or P2'-modified peptide substrates but unchanged specificity with P4-, P3-, P1, P1' and P3'-modified substrates	Rous sarcoma virus
I44V	mutant enzyme shows 211% of the activity with PPAVSLAMTMRR as substrate compared to activity of the wild-type enzyme. Mutant enzyme exhibits HIV-1 protease specificityfor P2- or P2'-modified peptide substrates but unchanged specificity with P4-, P3-, P1, P1' and P3'-modified substrates	Rous sarcoma virus
M73V	mutant enzyme shows 31% of the activity with PPAVSLAMTMRR as substrate compared to activity of the wild-type enzyme	Rous sarcoma virus
M73V/A100L	mutant enzyme shows 88% of the activity with PPAVSLAMTMRR as substrate compared to activity of the wild-type enzyme	Rous sarcoma virus
N61P/P62L/Q63M	mutant enzyme shows 434% of the activity with PPAVSLAMTMRR as substrate compared to activity of the wild-type enzyme	Rous sarcoma virus
Q63M	mutant enzyme shows 447% of the activity with PPAVSLAMTMRR as substrate compared to activity of the wild-type enzyme	Rous sarcoma virus
R105P/G106V	mutant enzyme shows 98% of the activity with PPAVSLAMTMRR as substrate compared to activity of the wild-type enzyme	Rous sarcoma virus
R105P/G106V/S107N	mutant enzyme shows 738% of the activity with PPAVSLAMTMRR as substrate compared to activity of the wild-type enzyme	Rous sarcoma virus
R105P/G106V/S107N	cleaves TSCYHCGT 50fold faster than the wild-type enzyme, cleaves PASSLAMT 140fold faster than the wild-type enzyme, cleaves PAVSLAMT 13.7fold faster than the wild-type enzyme, cleaves GAVSLAMT 48fold faster than the wild-type enzyme, cleaves PFVSLAMT 2.6fold faster than the wild-type enzyme	Rous sarcoma virus
S107N	mutant enzyme shows 370% of the activity with PPAVSLAMTMRR as substrate compared to activity of the wild-type enzyme	Rous sarcoma virus
V104T	mutant enzyme shows 229% of the activity with PPAVSLAMTMRR as substrate compared to activity of the wild-type enzyme	Rous sarcoma virus
V104T/R105P/G106V/S107N	mutant enzyme shows 496% of the activity with PPAVSLAMTMRR as substrate compared to activity of the wild-type enzyme	Rous sarcoma virus

Organism

Organism	UniProt	Comment	Textmining
Rous sarcoma virus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
PPAVSLAMTMRR + H2O	-	Rous sarcoma virus	PPAVS + LAMTMRR	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0183	-	PPAVSLAMTMRR	pH 5.9, mutant enzyme A100L	Rous sarcoma virus
0.0667	-	PPAVSLAMTMRR	pH 5.9, mutant enzyme M73V	Rous sarcoma virus
0.133	-	PPAVSLAMTMRR	pH 5.9, mutant enzyme I42D	Rous sarcoma virus
0.19	-	PPAVSLAMTMRR	pH 5.9, mutant enzyme M73V/A100L	Rous sarcoma virus
0.212	-	PPAVSLAMTMRR	pH 5.9, mutant enzyme R105P/G106V	Rous sarcoma virus
0.217	-	PPAVSLAMTMRR	pH 5.9, wild-type enzyme	Rous sarcoma virus
0.457	-	PPAVSLAMTMRR	pH 5.9, mutant enzyme I44V	Rous sarcoma virus
0.497	-	PPAVSLAMTMRR	pH 5.9, mutant enzyme V104T	Rous sarcoma virus
0.53	-	PPAVSLAMTMRR	pH 5.9, mutant enzyme DELTAN61-Q63	Rous sarcoma virus
0.817	-	PPAVSLAMTMRR	pH 5.9, mutant enzyme S107N	Rous sarcoma virus
0.94	-	PPAVSLAMTMRR	pH 5.9, mutant enzyme N61P/P62L/Q63M	Rous sarcoma virus
0.97	-	PPAVSLAMTMRR	pH 5.9, mutant enzyme Q63M	Rous sarcoma virus
1.08	-	PPAVSLAMTMRR	pH 5.9, mutant enzyme V104T/R105P/G106V/S107N	Rous sarcoma virus
1.6	-	PPAVSLAMTMRR	pH 5.9, mutant enzyme R105P/G106V/S107N	Rous sarcoma virus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)