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Literature summary for 3.4.23.B10 extracted from

  • Grinde, B.; Cameron, C.E.; Leis, J.; Weber, I.T.; Wlodawer, A.; Burstein, H.; Bizub, D.; Skalka, A.M.
    Mutations that alter the activity of the Rous sarcoma virus protease (1992), J. Biol. Chem., 267, 9481-9490.
    View publication on PubMed
Search for more literature for 3.4.23.B10

Protein Variants

Protein Variants Comment Organism
A40S mutant enzyme shows 23% of the activity compared to wild-type enzyme with PPAVSLAMTMRR Rous sarcoma virus
A40T mutant enzyme is inactive with PPAVSLAMTMRR Rous sarcoma virus
D37S mutant enzyme is inactive with PPAVSLAMTMRR Rous sarcoma virus
H65G mutant enzyme is inactive with PPAVSLAMTMRR Rous sarcoma virus
H65G/R105P/G106V mutant enzyme shows about 25% of the activity of the wild-type enzyme in RSV substrates, about 5fold higher activity with the HIV-1 peptide substrate PRKILFLDGRR Rous sarcoma virus
additional information if one of the surface loops is shortened, then the mutant exhibits only 2-3% of the activity associated with the wild-type enzyme Rous sarcoma virus
R105P/G106V mutant enzyme shows the same activity as the wild-type enzyme with all RSV substrates, 10fold higher activity with the HIV-1 peptide substrate PRKILFLDGRR Rous sarcoma virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 PPAVSLAMTMRR pH 5.9, 37°C, mutant enzyme R105P/G106V mutant enzyme Rous sarcoma virus
0.049
-
 PPAVSLAMTMRR pH 5.9, 37°C, AMV protease Rous sarcoma virus
0.077
-
 PPAVSLAMTMRR pH 5.9, 37°C, mutant enzyme H65G/R105P/G106V mutant enzyme Rous sarcoma virus
0.095
-
 PRKILFLDGRR pH 5.9, 37°C, mutant enzyme H65G/R105P/G106V Rous sarcoma virus
0.098
-
 PRKILFLDGRR pH 5.9, 37°C, AMV protease Rous sarcoma virus
0.1
-
 PRKILFLDGRR pH 5.9, 37°C, mutant enzyme R105P/G106V Rous sarcoma virus

Organism

Organism UniProt Comment Textmining
Rous sarcoma virus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information
-
 Rous sarcoma virus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
PPAVSLAMTMRR + H2O
-
 Rous sarcoma virus PPAVS + LAMTMRR
-
 ?
PRKILFLDGRR + H2O
-
 Rous sarcoma virus PRKIL + FLDGRR
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0217
-
 PRKILFLDGRR pH 5.9, 37°C, AMV protease Rous sarcoma virus
0.0917
-
 PRKILFLDGRR pH 5.9, 37°C, mutant enzyme H65G/R105P/G106V Rous sarcoma virus
0.102
-
 PPAVSLAMTMRR pH 5.9, 37°C, mutant enzyme H65G/R105P/G106V mutant enzyme Rous sarcoma virus
0.193
-
 PRKILFLDGRR pH 5.9, 37°C, mutant enzyme R105P/G106V Rous sarcoma virus
0.332
-
 PPAVSLAMTMRR pH 5.9, 37°C, mutant enzyme R105P/G106V Rous sarcoma virus
0.36
-
 PPAVSLAMTMRR pH 5.9, 37°C, AMV protease Rous sarcoma virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 A40S mutant enzyme, reaction with PPAVSLAMTMRR Rous sarcoma virus

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5: about 60% of maximal activity, pH 8: about 10% of maximal activity, A40S mutant enzyme, reaction with PPAVSLAMTMRR Rous sarcoma virus