Literature summary for 3.4.23.B10 extracted from

Ridky, T.W.; Bizub-Bender, D.; Cameron, C.E.; Weber, I.T.; Wlodawer, A.; Copeland, T.; Skalka, A.M.; Leis, J.
Programming the Rous sarcoma virus protease to cleave new substrate sequences (1996), J. Biol. Chem., 271, 10538-10544.

Search for more literature for 3.4.23.B10

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and histidine-tagged mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N are expressed in Escherichia coli M15 pDM1	Rous sarcoma virus

Crystallization (Commentary)

Crystallization (Comment)	Organism
the multiple substitution allow the protease to cleave four HIV-1 peptide substrates at initial rates more than 100fold greater than wild-type enzyme	Rous sarcoma virus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.006	-	PPAVSLAMTMRR	pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus
0.111	-	PARVLFLDGRR	pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus
0.132	-	PATIMMORERR	pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus
0.283	-	PGNFLQSRR	pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus
0.366	-	PARVLAEAMRR	pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus

Organism

Organism	UniProt	Comment	Textmining
Rous sarcoma virus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and mutant linked-dimer RSV protease	Rous sarcoma virus

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	recombinant enzyme is purified from inclusion bodies in Escherichia coli	Rous sarcoma virus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme is a homodimer which acts symmetrically in substrate selection with each enzyme subunit being capable of recognizing both halves of a peptide substrate equally	Rous sarcoma virus	?	-	?
PARVLAEAMRR + H2O	reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus	PARVL + AEAMRR	-	?
PARVLFLDGRR + H2O	reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus	PARVL + FLDGRR	-	?
PATIMMORERR + H2O	reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus	PATIM + MORERR	-	?
PGNFLQSRR + H2O	reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus	PGNF + LQSRR	-	?
PPAVSLAMTMRR + H2O	reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus	PPAVS + LAMTMRR	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.283	-	PGNFLQSRR	pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus
0.717	-	PPAVSLAMTMRR	pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus
1.63	-	PARVLAEAMRR	pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus
2.98	-	PATIMMORERR	pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus
8.2	-	PARVLFLDGRR	pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N	Rous sarcoma virus

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Release 2023.1 (January 2023)