Application | Comment | Organism |
---|---|---|
diagnostics | napsin A is an intracellular aspartic protease and biomarker of various malignancies like lung adenocarcinoma and ovarian clear cell carcinoma | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21 (DE3), recombinant overexpression in HEK-293 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin A | napsin A binds over a broad pH range to this inhibitor, highly selective binding of napsin A | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O96009 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | deglycosylation by PNGase F | Homo sapiens |
proteolytic modification | model of successive maturation, overview | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
lung | - |
Homo sapiens | - |
lung adenocarcinoma cell | - |
Homo sapiens | - |
additional information | napsin A is predominantly expressed in lung and kidney. Lung napsin A in many mammals is a heterogeneous enzyme with a characteristic ladder-like appearance in SDS-PAGE that might be caused by proteolytically processed N- and/or C-termini, in contrast to the more homogeneous form found in kidneys and primary lung adenocarcinoma | Homo sapiens | - |
ovarian clear cell carcinoma cell | - |
Homo sapiens | - |
ovary | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
napsin A | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | pepstatin pull-down at high pH is a powerful tool for detection and analysis of napsin A. Method development for specific enrichment of napsin A from clinical or biological specimens, that facilitates detailed analysis of the protein, evaluation, overview | Homo sapiens |
physiological function | napsin A is an intracellular aspartic protease and biomarker of various malignancies like lung adenocarcinoma and ovarian clear cell carcinoma | Homo sapiens |