Cloned (Comment) | Organism |
---|---|
expression in HEK-293 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
K(dabsyl)-TSLLMAAPQ-Lucifer yellow | pH 4.7, 25°C | Homo sapiens | |
0.0062 | - |
K(dabsyl)-TSVLMAAPQ-Lucifer yellow | pH 4.7, 25°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, deglycosylated protein, SDS-PAGE | Homo sapiens |
39000 | - |
x * 39000, glycosylated protein, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
K(dabsyl)-TSLLMAAPQ-Lucifer yellow + H2O | Homo sapiens | - |
K(dabsyl)-TSLL + MAAPQ-Lucifer yellow | - |
? | |
polypeptide + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O96009 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant from HEK-293 cells, to homogeneity, 1-step procedure with propeptide of napsin A as affinity ligand | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
K(dabsyl)-TSLLMAAPQ-Lucifer yellow + H2O | - |
Homo sapiens | K(dabsyl)-TSLL + MAAPQ-Lucifer yellow | - |
? | |
K(dabsyl)-TSVLMAAPQ-Lucifer yellow + H2O | - |
Homo sapiens | K(dabsyl)-TSVL + MAAPQ-Lucifer yellow | - |
? | |
polypeptide + H2O | - |
Homo sapiens | peptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, deglycosylated protein, SDS-PAGE | Homo sapiens |
? | x * 39000, glycosylated protein, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 5.5 | recombinant enzyme, at room temperature | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
10 h, 37°C, stable | Homo sapiens |
6 | - |
10 h, 37°C, stable | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000002 | - |
pepstatin | pH 4.7, 25°C | Homo sapiens |