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Literature summary for 3.4.23.B1 extracted from

  • Schauer-Vukasinovic, V.; Bur, D.; Kitas, E.; Schlatter, D.; Rosse, G.; Lahm, H.W.; Giller, T.
    Purification and characterization of active recombinant human napsin A (2000), Eur. J. Biochem., 267, 2573-2580.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK-293 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
pepstatin
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
K(dabsyl)-TSLLMAAPQ-Lucifer yellow pH 4.7, 25°C Homo sapiens
0.0062
-
K(dabsyl)-TSVLMAAPQ-Lucifer yellow pH 4.7, 25°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
x * 37000, deglycosylated protein, SDS-PAGE Homo sapiens
39000
-
x * 39000, glycosylated protein, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
K(dabsyl)-TSLLMAAPQ-Lucifer yellow + H2O Homo sapiens
-
K(dabsyl)-TSLL + MAAPQ-Lucifer yellow
-
?
polypeptide + H2O Homo sapiens
-
peptides
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O96009
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from HEK-293 cells, to homogeneity, 1-step procedure with propeptide of napsin A as affinity ligand Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
K(dabsyl)-TSLLMAAPQ-Lucifer yellow + H2O
-
Homo sapiens K(dabsyl)-TSLL + MAAPQ-Lucifer yellow
-
?
K(dabsyl)-TSVLMAAPQ-Lucifer yellow + H2O
-
Homo sapiens K(dabsyl)-TSVL + MAAPQ-Lucifer yellow
-
?
polypeptide + H2O
-
Homo sapiens peptides
-
?

Subunits

Subunits Comment Organism
? x * 37000, deglycosylated protein, SDS-PAGE Homo sapiens
? x * 39000, glycosylated protein, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4 5.5 recombinant enzyme, at room temperature Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
10 h, 37°C, stable Homo sapiens
6
-
10 h, 37°C, stable Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000002
-
pepstatin pH 4.7, 25°C Homo sapiens