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Literature summary for 3.4.23.52 extracted from
- Cleavage of preflagellins by an aspartic acid signal peptidase is essential for flagellation in the archaeon Methanococcus voltae (2003), Mol. Microbiol., 50, 1339-1347.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in BL21(DE3)/pLysS membranes | Methanococcus voltae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D186A | the mutation does not affect the activity towards preflagellin FlaB2 | Methanococcus voltae |
| D18A | inactive | Methanococcus voltae |
| D18E | the mutation results in an apparently unstable protein | Methanococcus voltae |
| D18N | inactive | Methanococcus voltae |
| D190A | the mutation does not affect the activity towards preflagellin FlaB2 | Methanococcus voltae |
| D224A | the mutation does not affect the activity towards preflagellin FlaB2 | Methanococcus voltae |
| D79A | inactive | Methanococcus voltae |
| D79E | the mutant restores preflagellin peptidase activity (despite a reduced amount of peptidase produced) | Methanococcus voltae |
| D79N | inactive | Methanococcus voltae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Methanococcus voltae | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25900 | - |
x * 25900, calculated from amino acid sequence | Methanococcus voltae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanococcus voltae | - |
- |
- |
| Methanococcus voltae PS | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| preflagellin FlaB2 + H2O | - |
Methanococcus voltae | flagellin FlaB2 + ? | - |
? |  |
| preflagellin FlaB2 + H2O | - |
Methanococcus voltae PS | flagellin FlaB2 + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25900, calculated from amino acid sequence | Methanococcus voltae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FlaK | - |
Methanococcus voltae |
| RMVO00262 | recombinant enzyme | Methanococcus voltae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme plays an essential role in flagellation | Methanococcus voltae |
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Release 2023.1 (January 2023)
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