Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli K-12 |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 28% (w/v) polyethylene glycol 400, 0.2 M CaCl2, and 0.1 M Na-HEPES (pH 7.5), at 20°C | Escherichia coli K-12 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
- |
Escherichia coli K-12 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | P0AEV9 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap HP5 column chromatography, HiPrep column chromatography, and Superdex 200 gel filtration | Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
precursor of the large subunit of hydrogenase 3 + H2O | the HycI endopeptidase is involved in the C-terminal processing of the large subunit of hydrogenase 3 (HycE) | Escherichia coli K-12 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 17000, HycI exists as a monomer in solution, X-ray crystallography | Escherichia coli K-12 |
Synonyms | Comment | Organism |
---|---|---|
HycI endopeptidase | - |
Escherichia coli K-12 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli K-12 | calculated from amino acid sequence | - |
3.7 |