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Literature summary for 3.4.23.5 extracted from
- Cathepsin D from the hepatopancreas of the cuttlefish (Sepia officinalis): purification and characterization (2010), J. Agric. Food Chem., 58, 10623-10630.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | 385.5% activity at 1 mM | Sepia officinalis |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 54% residual activity at 5 mM | Sepia officinalis | |
| E-64 | 83% residual activity at 1 mM | Sepia officinalis | |
| Fe2+ | 68% residual activity at 5 mM | Sepia officinalis | |
| Fe3+ | 12.4% residual activity at 5 mM | Sepia officinalis | |
| Hg2+ | 26.2% residual activity at 5 mM | Sepia officinalis | |
| iodoacetic acid | 76% residual activity at 1 mM | Sepia officinalis | |
| Mn2+ | 32.5% residual activity at 5 mM | Sepia officinalis | |
| molybdate | 2.3% residual activity at 1 mM | Sepia officinalis | |
| additional information | no inhibition with phenylmethylsulfonyl fluoride, benzamidine, soybean trypsin inhibitor, TPCK, and EDTA | Sepia officinalis | |
| pepstatin A | completely inhibited by 0.0015 mM pepstatin A | Sepia officinalis | |
| SDS | 28% residual activity at 0.2% (w/v) | Sepia officinalis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 191.3% activity at 5 mM | Sepia officinalis | |
| Co2+ | 239.4% activity at 5 mM | Sepia officinalis | |
| Cu2+ | 157% activity at 5 mM | Sepia officinalis | |
| Mg2+ | 137.6% activity at 5 mM | Sepia officinalis | |
| additional information | cathepsin D is not affected by Na+, K+, and Ca2+ | Sepia officinalis | |
| Ni2+ | 176% activity at 5 mM | Sepia officinalis | |
| Sr2+ | 212.5% activity at 5 mM | Sepia officinalis | |
| Zn2+ | 123.7% activity at 5 mM | Sepia officinalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37500 | - |
x * 37500, purified enzyme, estimated from SDS-PAGE | Sepia officinalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sepia officinalis | - |
cuttlefish | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Sephadex G-100 gel filtration, Mono-S column chromatography, Sephadex G-75 gel filtration, and Mono-S column chromatography | Sepia officinalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatopancreas | - |
Sepia officinalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the specific activity of the enzyme from crude extract and after 54.21fold purification in 100 mM glycine-HCl buffer, pH 3.0, at 50°C using hemoglobin as substrate are 6.63 units/mg and 359.44 units/mg, respectively. One unit of enzymatic activity against hemoglobin is defined as the amount of enzyme that catalyzes an increase of 1.0 in the absorbance at 280 nm per minute | Sepia officinalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| actin + H2O | - |
Sepia officinalis | ? | - |
? | |
| Bovine serum albumin + H2O | 21.4% activity compared to hemoglobin | Sepia officinalis | ? | - |
? | |
| casein + H2O | 46.3% activity compared to hemoglobin | Sepia officinalis | ? | - |
? | |
| gamma-globulin + H2O | 2.6% activity compared to hemoglobin | Sepia officinalis | ? | - |
? | |
| Gelatin + H2O | 12.9% activity compared to hemoglobin | Sepia officinalis | ? | - |
? | |
| Hemoglobin + H2O | 100% activity | Sepia officinalis | ? | - |
? | |
| additional information | no activity towards ovalbumin | Sepia officinalis | ? | - |
? | |
| myofibrillar protein + H2O | - |
Sepia officinalis | ? | - |
? | |
| myosin heavy chain + H2O | - |
Sepia officinalis | ? | - |
? | |
| paramyosin + H2O | - |
Sepia officinalis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37500, purified enzyme, estimated from SDS-PAGE | Sepia officinalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Sepia officinalis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 55 | about 10% activity at 20°C, about 20% activity at 30°C, about 40% activity at 40°C, about 60% activity at 45°C, 100% activity at 50°C, about 80% activity at 55°C, about 60% activity at 60°C, about 35% activity at 65°C, and about 10% activity at 70°C | Sepia officinalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 45 | the enzyme is highly stable at temperatures below 45°C but activity declines rapidly at temperatures exceeding 50°C no activity is detected after incubation at 65°C for 60 min | Sepia officinalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
- |
Sepia officinalis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 5 | the relative activities at pH 2.0, 4.0, and 5.0 are about 57, 65.7, and 23%, respectively, of that at pH 3.0. Cathepsin D activity decreases significantly above pH 5.0 and is only 2% at pH 6.0 | Sepia officinalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 5 | the enzyme maintains 100% of its original activity at pH 3.0 and 4.0 after 60 min of incubation at 25°C. At pH 2.0 and 5.0, the enzyme retains 41.0% and 97.3% of its initial activity, respectively | Sepia officinalis |
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