Application | Comment | Organism |
---|---|---|
molecular biology | insights on the amino acid region involved in the terminal processing of human cathepsin D and on the function of the processing beta-hairpin loop are provided | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a mutant cathepsin D protein is generated replacing the stretch from T155 to K175 (comprising the beta-hairpin loop) with the corresponding sequence S152-V159 of Clupea harengus. The mutant is successfully expressed in rodent cells (Neuro2A, L929 and DLD1 cells), reaches the lysosome and is stable as a single-chain peptide (47 kDa), bears high-mannose type sugars, binds to pepstatin A and is enzymatically active, indicating that it is correctly folded | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin A | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
intermediate, single-chain CD | Homo sapiens |
53000 | - |
precursor | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | human cathepsin D is synthesized as a di-glycosylated precursor of approximately 53 kDa that is processed by proteolysis first into an intermediate single-chain of 48 kDa and eventually into the mature double-chain form | Homo sapiens |
Purification (Comment) | Organism |
---|---|
using Pepstatin A-affinity purification | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine hemoglobin + H2O | conjugated to the fluorochrome 6-((7-amino-4-methylcoumarin-3-acetyl) amino) hexanoic acid, succinimidyl ester | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cathepsin D | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.6 | 5 | assay at | Homo sapiens |