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Literature summary for 3.4.23.5 extracted from
- Cathepsin D of rat spleen. Affinity purification and properties of two types of cathepsin D (1979), Eur. J. Biochem., 95, 459-467.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | - |
Rattus norvegicus |  |
| Pb2+ | cathepsin D-II inhibited, cathepsin D-I hardly affected | Rattus norvegicus | |
| PCMB | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | slight activation of cathepsin D-II | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | cathepsin D-I: neutral sugar content 6%, contains mannose, glucose, galactose, fucose and glucosamine in a ratio of 8:2:1:1:5 | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity purification, 2 enzyme forms, the major form is termed cathepsin D-1, the minor form is termed cathepsin D-II | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spleen | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Albumin + H2O | bovine serum albumin | Rattus norvegicus | ? | - |
? | |
| Albumin + H2O | human serum albumin | Rattus norvegicus | ? | - |
? | |
| Hemoglobin + H2O | - |
Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 44000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
cathepsin D-II | Rattus norvegicus |
| 55 | - |
cathepsin D-I | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
pH 7.0, 90 min, 40% loss of activity | Rattus norvegicus |
| 60 | - |
10 min, pH 3.8 or pH 7.0, loss of a large portion of activity of enzyme form cathepsin D-I and cathepsin D-II | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | - |
10 min, 60°C, loss of a large portion of activity of enzyme form cathepsin D-I and cathepsin D-II | Rattus norvegicus |
| 7 | - |
10 min, 60°C, loss of a large portion of activity of enzyme form cathepsin D-I and cathepsin D-II | Rattus norvegicus |
| 7 | - |
37°C, 90 min, 40% loss of activity | Rattus norvegicus |
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Release 2023.1 (January 2023)
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