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Literature summary for 3.4.23.49 extracted from
- Identification and antibody-therapeutic targeting of chloramphenicol-resistant outer membrane proteins in Escherichia coli (2007), J. Proteome Res., 6, 3628-3636.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | studies on treatment of infection by antibiotic-resistant bacteria | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| inserted into pET-32a, expressed in Escherichia coli | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| outer membrane | - |
Escherichia coli | 19867 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35540 | - |
mass spectrometry | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P09169 | K12 BW25113 and DELTA-ompT | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, gel filtration and SDS-PAGE | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
comparative proteome analysis of chloramphenicol-resistant Escherichia coli, two-dimensional electrophoresis, MALDI-TOF mass spectroscopy and Western blotting performed, differential protein expression profiles with and without chloramphenicol treatment shown, antimicrobial susceptibility tested, OmpT protease identified as critically altered protein in chloramphenicol-resistant Escherichia coli, mutant analysis performed | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | OmpT shown to be one of the critical outer membrane protein responsible for chloramphenicol resistance, analysis by comparative proteomics and mutant investigation | Escherichia coli | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OmpT protease | - |
Escherichia coli |
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