Application | Comment | Organism |
---|---|---|
medicine | potential target for chemotherapy of virus infection and associated diseases, essential for maturation of infectious virions, development of drugs against the protease should be effective against HIV-2 | Human immunodeficiency virus 2 |
Cloned (Comment) | Organism |
---|---|
cloned and expressed in Escherichia coli JM-101, F' and X90 | Human immunodeficiency virus 2 |
Crystallization (Comment) | Organism |
---|---|
crystallized by hanging drop method in complex with a reduced amide inhibitor, BI-LA-398 | Human immunodeficiency virus 2 |
General Stability | Organism |
---|---|
activity is lost during purification and storage | Human immunodeficiency virus 2 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
NH2-ATLNFPSPW-COOH | pH 5.5, 37°C | Human immunodeficiency virus 2 | |
0.95 | - |
NH2-ATLNFPSPW-COOH | pH 5.5, 37°C, mutant K7Q | Human immunodeficiency virus 2 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GAG precursor protein of HIV-2 + H2O | Human immunodeficiency virus 2 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 2 | - |
mutant K7Q | - |
Human immunodeficiency virus 2 | - |
human immunodeficiency virus type 2 | - |
Purification (Comment) | Organism |
---|---|
mutant K7Q, autoproteolysis | Human immunodeficiency virus 2 |
recombinant enzyme | Human immunodeficiency virus 2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GAG precursor protein of HIV-2 + H2O | - |
Human immunodeficiency virus 2 | ? | - |
? | |
additional information | cleavage sites identified by amino-terminal sequence analysis are F3-S4, Y14-I15, G35-I36, G52-F53, M76-T77, L90-T91 | Human immunodeficiency virus 2 | ? | - |
? | |
NH2-ATLNFPSPW-COOH + H2O | - |
Human immunodeficiency virus 2 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
NH2-ATLNFPSPW-COOH | pH 5.5, 37°C | Human immunodeficiency virus 2 | |
4.33 | - |
NH2-ATLNFPSPW-COOH | pH 5.5, 37°C, mutant K7Q | Human immunodeficiency virus 2 |